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Plant regeneration from protoplasts of Dimorphotheca and Rudbeckia

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Abstract

Protoplasts were isolated from leaves, shoots, cotyledons, ray florets and callus cultures of Dimorphotheca aurantiaca (syn. D. sinuata) (Cape Marigold, Star of the Veldt) and Rudbeckia hirta, R. laciniata and R. purpurea; species of ornamental value. For Dimorphotheca, plants were regenerated from protoplasts of all sources apart from the ray floret, whilst for the Rudbeckia species, although protoplast division was induced in most cases, only leaf mesophyll protoplasts of R. hirta c.v. Marmalade gave plants. The establishment of plant regeneration for these ornamental species, from protoplasts, now provides a basis for their somatic hybridisation.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

NAA:

naphthaleneacetic acid

K:

kinetin

GA3 :

gibberellic acid

MS:

Murashige and Skoog (1962)

f.wt.:

fresh weight

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    J. S. Al-Atabee & J. B. Power

Authors
  1. J. S. Al-Atabee
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  2. J. B. Power
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Communicated by I. Potrykus

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Al-Atabee, J.S., Power, J.B. Plant regeneration from protoplasts of Dimorphotheca and Rudbeckia . Plant Cell Reports 6, 414–416 (1987). https://doi.org/10.1007/BF00272771

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  • DOI: https://doi.org/10.1007/BF00272771

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