Abstract
Leaf, stem and root explants of Mandevilla velutina were cultured in vitro and produced vigorous callus in LS basal medium containing one auxin (2,4-D or NAA) plus BAP. Calli can be subcultured indefinitely with vigorous growth. Subculture of calli to NAA (1.0 mg/l) plus BAP (5.0 mg/l) caused profuse regeneration of shoots. Isolated shoots were rooted in basal medium plus NAA (5.0 mg/l) or IBA (8.0 mg/l). Rapidly growing cell suspensions can be easily obtained from friable callus cultured in liquid medium.
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Abbreviations
- LS:
-
Linsmaier & Skoog
- 2,4-D:
-
2,4 dichlorophenoxi-acetic acid
- NAA:
-
α-naphthalene-acetic acid
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- BAP:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
References
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Communicated by I. K. Vasil
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Handro, W., Floh, E.I.S., Ferreira, C.M. et al. Tissue, cell culture and micropropagation of Mandevilla velutina, a natural source of a bradykinin antagonist. Plant Cell Reports 7, 564–566 (1988). https://doi.org/10.1007/BF00272758
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DOI: https://doi.org/10.1007/BF00272758