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Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.)

Plant Cell Reports volume 7, pages 553–556 (1988)Cite this article

Abstract

Callus tissues derived from seedlings of roselle (Hibiscus sabdariffa L.) were shown to produce two cyanidin glycosides as major anthocyanin pigments. Both callus growth and anthocyanin synthesis were remarkably stimulated by 2,4-dichlorophenoxyacetic acid. The highest anthocyanin yield was observed when 1 μM 2,4-D in combination with 0.1–1 μM kinetin was supplemented to the culture medium. In contrast, gibberellic acid showed inhibitory effect on anthocyanin production.

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Abbreviations

LS:

Linsmaier and Skoog

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

GA:

gibberellic acid

References

  • Cheng CL, Wetherell, DF, Dougall DK (1985) In: Neumann KH, Barz W, Reinhard E (ed) Primary and Secondary Metabolism in Plant Cell Cultures, Springer, Berlin Heiderberg New York, pp 87–98

    Google Scholar 

  • Clydesdale FM, Main JH, Francis, FJ (1979) J. Food Prot. 42:204–207

    Google Scholar 

  • Constabel F, Shyluk JP, Gamborg OL (1971) Planta 96:306–316

    Google Scholar 

  • Du DT, Francis FJ (1973) J. Food Sci. 38:810–812

    Google Scholar 

  • Gregor HD (1974) Protoplasma 80:273–277

    Google Scholar 

  • Hall RD, Yeoman MM (1986) New Phytol. 103:33–43

    Google Scholar 

  • Harborne JB (1958) Biochem J. 70:22–29

    Google Scholar 

  • Harborne JB (1967) Comparative Biochemistry of the Flavonoids. Academic Press, New York

    Google Scholar 

  • Harborne JB (1984) Phytochemical Methods— 2nd ed. Chapmann and Hall, London

    Google Scholar 

  • Heinzmann U, Seitz U (1977) Planta 135:63–67

    Google Scholar 

  • Hinderer W, Petersen M, Seitz U (1984) Planta 160:544–549

    Google Scholar 

  • Leweke B, Forkmann G (1982) Plant Cell Rep. 1:98–100

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127

    Google Scholar 

  • Nozue M, Kawai J, Yoshitama K (1987) J. Plant Physiol. 129:81–88

    Google Scholar 

  • Ozeki Y, Komamine A (1986) Plant Cell Physiol. 27:1361–1368

    Google Scholar 

  • Rau D, Forkmann G (1986) Plant Cell Rep. 5:435–438

    Google Scholar 

  • Schmitz M, Seitz U (1972) Z. Pflanzenphysiol. 68: 259–265

    Google Scholar 

  • Shibata M, Furukawa M (1969) Bot. Mag. Tokyo 82:341–347

    Google Scholar 

  • Smith SL, Slywka W, Kruger RJ (1981) J. Nat. Prod. 44:609–610

    Google Scholar 

  • Yamakawa T, Ishida K, Kato S, Kodama T (1983a) Agric. Biol. Chem. 47:997–1001

    Google Scholar 

  • Yamakawa T, Kato S, Ishida S, Kodama I, Minoda Y (1983b) Agric. Biol. Chem. 47:2185–2191

    Google Scholar 

Download references

Author information

Affiliations

  1. Faculty of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan

    Hajime Mizukami, Kaomi Tomita & Hiromu Ohashi

  2. Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, 950-21, Niigata, Japan

    Noboru Hiraoka

Authors
  1. Hajime Mizukami
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  2. Kaomi Tomita
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  3. Hiromu Ohashi
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  4. Noboru Hiraoka
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Additional information

Communicated by F. Constabel

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Mizukami, H., Tomita, K., Ohashi, H. et al. Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.). Plant Cell Reports 7, 553–556 (1988). https://doi.org/10.1007/BF00272755

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  • DOI: https://doi.org/10.1007/BF00272755

Keywords

  • Glycoside
  • Kinetin
  • Gibberellic Acid
  • Callus Culture
  • Callus Tissue