Summary
The shift of recessive suppressor mutant of yeast Saccharomyces cerevisiae from permissive to restrictive conditions is accompanied by polysome decay and accumulation of 80 S ribosomes (Smirnov et al., 1976). In this paper some properties of 80 S ribosomes are studied. It is demonstrated that polysome decay under non-permissive conditions is not the consequence of the impairement of RNA synthesis. More than 70% of 80 S ribosomes accumulated under non-permissive conditions contain bound peptidyl-tRNAs localized in P-ribosomal site. tRNA moiety of bound peptidyl-tRNA is able to accept all 20 natural amino acids after chemical deacylation. Therefore it is not a specific isoacceptor species but rather total tRNA that is bound to ribosomes. The polypeptide residues of these peptidyl-tRNAs are heterogeneous in size. Their molecular weights are comparable with the molecular weights of the completed polypeptides. Some of the 80 S ribosomes accumulated under non-permissive conditions contain poly-A RNA. In conclusion, possible mechanism of the impairement of translation under non-permissive conditions in recessive suppressor strain is discussed.
Similar content being viewed by others
References
Cardelli, J., Pilot, H.C.: Isolation and characterization of rat liver free and membrane-bound polysomal messenger ribonucleoprotein particles. Biochemistry 16, 5127–5134 (1977)
Gerlach, W.L.: Genetic properties of some amber-ochre supersuppressors in Saccharomyces cerevisiae. Mol. Gen. Genet. 138, 53–63 (1975)
Gerlach, W.L.: Mutational properties of Sup P amber-ochre supersuppressors in Saccharomyces cerevisiae. Mol. Gen. Genet 144, 213–215 (1976)
Gesteland, J.F., Wolfnor, M., Grisafi, P., Fink, G., Botstein, D., Roth, J.R.: Yeast suppressors of UAA and UAG nonsense condons work effeciently in vitro via tRNA. Cell 7, 381–390 (1976)
Hartwell, L.H., McLaughlin, C.S.: Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J. Bacteriol. 96, 1664–1671 (1968)
Hawthorn, D.C., Leupold, U.: Suppressor mutations in yeast. Curr. Top. Microbiol. Immunol. 64, 1–47 (1974)
Inge-Vechtomov, S.G., Andrianova, V.M.: Recessive supersuppressors in yeast (in Russian). Genetika (USSR) 11, 103–115 (1970)
Inge-Vechtomov, S.G.: Suppressors of adenine requirement in yeast (in Russian). Genetika (USSR) 2, 22–26 (1975)
Jimenez, A., Carrasco, L., Vazquez, D.: Enzymic and nonenzymic translocation by yeast polysomes. Site of action of a number of inhibitors. Biochemistry 16, 4227–4730 (1970)
Kabat, D.: Direct method of measuring transfer ribonucleic acid binding to polyribosomes and to single ribosomes in cells of higher organisms. Anal. Biochem. 39, 228–234 (1971)
Lengyel, P.: The process of translation: a bird's eye view in ribosomes (Nomura M., ed.), pp. 13–52. New York: Cold Spring Harbor Laboratory 1974
Mager, W.H., Planta, R.J.: Yeast ribosomal proteins are synthesized on small polysomes. Europ. J. Biochem. 62, 193–197 (1976)
Mortimer, R.K., Hawthorne, D.C.: Genetic mapping of nonsense suppressors in yeast. Genetics 60, 735–740 (1968)
Peterson, N.S., McLaughlin, C.S.: Polysome metabolism in protein synthesis mutants of yeast. Mol. Gen. Genet. 129, 189–200 (1974)
Plapp, F.P., Hayes, L.C., Tilzer, L., Chiga, M.: Dissociability and tRNA content of ribosomes produced by dimethylnitrosamine and starvation. Nature 247, 311–313 (1974)
Smirnov, V.N., Kreier, V.G., Lizlova, L.V., Andrianova, V.M., Inge-Vechtomov, S.G.: Recessive super-suppression in yeast. Mol. Gen. Genet. 129, 105–121 (1974)
Smirnov, V.N., Surguchov, A.P., Fominykch, E.S., Lizlova, L.V., Saprygina, T.V., Inge-Vechtomov, S.G.: Recessive nonsensesuppression in yeast: further characterization of a defect in translation. FEBS Lett. 66, 12–15 (1976)
Smirnov, V.N., Surguchov, A.P., Smirnov, V.V., Berestetskaya, Yu. V., Inge-Vechtomov, S.G.: Recessive nonsense-suppression in yeast: involvement of 60 S ribosomal subunit. Mol. Gen. Genet. 163, 87–90 (1978)
Stewart, J.W., Sherman, F.: Demonstration of UAG nonsense codons in bakers yeast by amino acid replacements in iso-I-cytochrom c. J. Mol. Biol. 68, 429 (1972)
Surguchov, A.P., Fominykch, E.S., Lizlova, L.V.: Dissociability of free and peptidyl-tRNA bound ribosomes. Mol. Biol. Rep. 4, 79–81 (1978)
Surguchov, A.P., Fominykch, E.S., Pospelova, E.M., Smirnov, V.N., Inge-Vechtomov, S.G., A recessive suppressors strain of yeast Saccharomyces cerevisiae with high level of ribosomal subparticles. (In press, FEBS Letters, 1979)
Van der Zeijst Ben, A.M., Kool, A.J., Bloemers, H.P.J.: Isolation of active ribosomal subunits from yeast. Europ. J. Biochem. 30, 15–25 (1972)
Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406–4412 (1969)
Author information
Authors and Affiliations
Additional information
Communicated by D. Goldfarb
Rights and permissions
About this article
Cite this article
Surguchov, A.P., Fominykch, E.S., Berestetskaya, Y.V. et al. Recessive nonsense-suppression in yeast Saccharomyces cerevisiae . Molec. Gen. Genet. 177, 675–680 (1980). https://doi.org/10.1007/BF00272679
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272679