Summary
The adaptive-response is an inducible repair system of E. coli which reduces the mutagenic and cytotoxic effects of alkylation damage (Samson and Cairns, 1977). In adapted cells (cells exposed to sublethal doses of alkylating agents) the induction of W-reactivation and W-mutagenesis by alkylating agents is almost totally blocked. Despite the fact that adaptation has no detectable effect on UV mutagenesis in E. coli K-12, it does inhibit to some extent the UV and tif-1 mediated induction of SOS functions such as W-reactivation and λ prophage induction. Furthermore, the kinetics of induction of W-mutagenesis following UV treatment are altered by adaptation. In this case the adaptive-response seems to specifically block the induction of an error-producing W-reactivating capacity which normally would increase soon after UV treatment, while affecting error-free W-reactivating systems to a lesser extent.
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Bresler, S.E., Kalinin, V.L., Shelegedin, V.N.: W-reactivation and W-mutagenesis of gamma irradiated phage lambda. Mutat. Res. 49, 341–355 (1978)
Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli. I. Further characterization of a thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Mol. Gen. Genet. 119, 139–152 (1972)
Defais, M., Caillet-Fauquet, P., Fox, M.S., Radman, M.: Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation. Mol. Gen. Genet. 148, 125–130 (1976)
Defais, M., Fauquet, P., Radman, M., Errera, M.: Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43, 495–503 (1971)
Elespuru, R.K., Yarmolinsky, M.B.: A colorimetric assay of lysogenic induction designed for screening potential carcinogenic and carcinostatic agents. Environ. Mut. 1, 65–78 (1979)
Emmerson, P.T., West, S.C.: Identification of protein X of Escherichia coli as the recA+/tif+ gene product. Mol. Gen. Genet. 155, 77–85 (1977)
George, J., Castellazzi, M., Buttin, G.: Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit, the expression of mutator properties of tif. Mol. Gen. Genet. 140, 309–332 (1975)
Gudas, L., Mount, D.: Identification of the recA (tif) gene product of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 74, 5280–5284 (1977)
Howard-Flanders, P., Boyce, R.P.: DNA repair and gene recombination: studies of mutants of Escherichia coli defective in these processes. Radiat. Res. Suppl. 6, 156–184 (1966)
Howard-Flanders, P., Boyce, R.P., Theriot, L.: Three loci in Escherichia coli K12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53, 1113–1136 (1966)
Ichikawa-Ryo, H., Kondo, S.: Indirect mutagenesis in phage lambda by ultraviolet preirradiation of host bacteria. J. Mol. Biol. 97, 77–92 (1975)
Jeggo, P., Defais, M. Samson, L., Schendel, P.: An adaptive response of E. coli to low levels of alkylating agents: comparison with previously characterized DNA repair pathways. Mol. Gen. Genet. 157, 1–9 (1977)
Lederberg, E.: Genetic and functional aspects of galactose metabolism in Escherichia coli K12. Soc. Gen. Micro. Symp. 11, 115–131 (1960)
McEntee, K.: Protein X is the product of the recA gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 74, 5275–5279 (1977)
Mattern, I.E., Van Winden, M.P., Rörsch, A.: The range of action of genes controlling radiation sensitivity of Escherichia coli. Mutat. Res. 2, 111–131 (1965)
Miura, A., Tomizawa, J.I.: Studies on radiation sensitive mutants of E. coli III. Participation of the rec system in induction of mutation by ultraviolet irradiation. Mol. Gen. Genet. 103, 1–10 (1968)
Monk, M., Kinross, J.: The kinetics of derepression of prophage λ following ultraviolet irradiation of lysogenic cells. Mol. Gen. Genet. 137, 263–268 (1975)
Mount, D.W., Kosel, C.K., Walker, A.: Inducible error free DNA repair in tsl recA mutants of E. coli Mol. Gen. Genet. 146, 37–41 (1976)
Radman, M.: Phenomenology of an inducible mutagenic DNA repair pathway in E. coli.: SOS repair hypothesis. In: Molecular and environmental aspects of mutagenesis (Prakash, L., Sherman, F., Miller, M.W., Lawrence, C.W., Taber, H.W., eds.), pp. 128–142. Springfield, Illinois: C.C. Thomas Publ. 1974
Radman, M.: SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. In: Molecular mechanisms for repair of DNA (Hanawalt P.C. and Setlow, R.B., eds.), pp. 355–367. New York, NY: Plenum Press 1975
Roberts, J.W., Roberts, C.W., Mount, D.W.:Inactivation and proteolytic cleavage of phage λ repressor in vitro in an ATP-dependent reaction. Proc. Natl. Acad. Sci. U.S.A. 74, 2283–2287 (1977)
Roberts, J.W., Roberts, C.W.: Proteolytic cleavage of bacteriophage lambda repressor in induction. Proc. Natl. Acad. Sci. U.S.A. 72, 147–151 (1975)
Robins, P., Cairns, J.: Quantitation of the adaptive response to alkylating agents. Nature 280, 74–76 (1979)
Rothman, R.H., Margossian, L.J., Clark, A.J.: W-reactivation of phage lambda in recF, uvrA and uvrB mutants of E. coli K12. Mol. Gen. Genet. 169, 279–287 (1979)
Samson, L., Cairns, J.: A new pathway for DNA repair in Escherichia coli. Nature 267, 281–283 (1977)
Schendel, P.F., Defais, M., Jeggo, P., Samson, L., Cairns, J.: Pathways of mutagenesis and repair in Escherichia coli exposed to low levels of simple alkylating agents. J. Bacteriol. 135, 466–475 (1978a)
Schendel, P.F., Defais, M., Jeggo, P., Samson, L., Cairns, J.: Pathways involved in repair of alkylation damage in E. coli. In: DNA Repair mechanisms (Hanawalt, P.C., Friedberg, E., Fox, C.F., eds.), ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. IX, 391–394 (1978b)
Schendel, P.F., Robins, P.: Repair of O6-methylguanine in adapted Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 75, 6017–6020 (1978)
Smith, C.L., Oishi, M.: Early events and mechanisms in the induction of bacterial SOS functions: Analysis of the phage repressor inactivation process in vivo. Proc. Natl. Acad. Sci. U.S.A. 75, 1657–1661 (1978)
Thomas, R.: Control of development in temperate bacteriophage. I. Induction of prophage gene following hetero-immune superinfection. J. Mol. Biol. 22, 79–95 (1966)
Tomizawa, J.I., Ogawa, T.: Effect of UV irradiation on bacteriophage λ immunity. J. Mol. Biol. 23, 247–263 (1967)
Weigle, J.S.: Induction of mutation in a bacterial virus. Proc. Natl. Acad. Sci. U.S.A. 39, 628–636 (1953)
Witkin, E.M.: Time, temperature and protein synthesis: a study of ultraviolet induced mutation in bacteria. Cold Spring Harbor Symp. Quant. Biol.21, 123–137 (1956)
Witkin, E.M.: Ultraviolet mutagenesis and inducible DNA repair in E. coli Bacteriol. Rev. 40, 869–907 (1976)
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Communicated by B.A. Bridges
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Defais, M., Jeggo, P., Samson, L. et al. Effect of the adaptive response on the induction of the SOS pathway in E. coli K-12. Molec. Gen. Genet. 177, 653–659 (1980). https://doi.org/10.1007/BF00272676
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DOI: https://doi.org/10.1007/BF00272676