Summary
We have followed the segregation of the probes pJ3.11, 7C22, pB79a, and MET through cystic fibrosis families in the German Democratic Republic with two affected sibs. Two families with a crossover between MET and the CF phenotype were detected. In one of these families recombination was also observed between the DNA probe 7C22 and CF, and between the markers XV-2c and CF, which suggests that XV-2c, MET and 7C22 are all on the same side of CF. The other MET recombinant family is informative with XV-2c and does not recombine, which excludes the genetic order XC-2c-MET-CF if multiple recombinant events are disregarded. These two families together demonstrate that recombinations may occur in a very small genetic interval, which has important implications for prenatal diagnosis based on data from linked markers.
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Berger, W., Hein, J., Gedschold, J. et al. Crossovers in two German cystic fibrosis families determine probe order for MET, 7C22 and XV-2c/CS.7. Hum Genet 77, 197–199 (1987). https://doi.org/10.1007/BF00272392
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DOI: https://doi.org/10.1007/BF00272392