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Construction of a chromosome 16-enriched phage library and characterization of several DNA segments from 16p

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Summary

A flow sorted chromosome 16-enriched recombinant library was produced to isolate DNA probes useful for constructing a linkage map of 16p, primarily for the study of adult polycystic kidney disease (APKD). The APKD locus has been mapped to chromosome 16 by linkage with the probe 3′HVR, which is located in the region 16p12→pter. Of the 48 single-copy fragments isolated from this new phage library, 39 (81%) were found to be chromosome 16 specific. Probes mapping to chromosome 16 were regionally localized by hybridizing to flow-sorted spot blots of translocation products from lymphoblastoid cell lines containing the rearrangements t(1;16) or t(11;16). Translocation breakpoints at 16p13.11 and 16p11.1 were utilized to subdivide chromosome 16 into three regions: Twenty-six probes were mapped to 16p11.1→16qter, two to 16p11.1→16p13.11, and eleven to 16p13.11→16pter. Probes from 16p were examined for their recognition of restriction fragment length polymorphisms (RFLPs). Seven polymorphic probes were found which recognized eleven RFLPs. Six of the seven probes have RFLPs which are reasonably informative (polymorphism information contents (PIC) of over 0.25). Two of these identify polymorphisms with three different alleles, one of which has a PIC value of over 0.4. These probes may aid in the diagnosis of APKD and contribute towards a linkage map of chromosome 16.

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  1. Marc Lalande

    Present address: Biotechnology Research Institute, Royal Victoria Hospital, H3A 1A1, Montreal, Canada

Authors and Affiliations

  1. Division of Genetics, Mental Retardation Center, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA

    Peter Harris, Marc Lalande, Helene Stroh, Gail Bruns, Alan Flint & Samuel A. Latt

  2. Howard Hughes Medical Institute, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA

    Peter Harris, Helene Stroh, Alan Flint & Samuel A. Latt

  3. Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA

    Peter Harris, Marc Lalande, Gail Bruns & Samuel A. Latt

  4. Department of Genetics, Harvard Medical School, 02115, Boston, MA, USA

    Samuel A. Latt

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  1. Peter Harris
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  5. Alan Flint
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Harris, P., Lalande, M., Stroh, H. et al. Construction of a chromosome 16-enriched phage library and characterization of several DNA segments from 16p. Hum Genet 77, 95–103 (1987). https://doi.org/10.1007/BF00272372

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  • DOI: https://doi.org/10.1007/BF00272372

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