Abstract
The glucan elicitor from cell walls of the fungal pathogen, Phytophthora megasperma f. sp. glycinea, induced rapid but transient increases in enzyme activities of general phenylpropanoid metabolism (phenylalanine ammonia-lyase and 4-coumarate: CoA ligase) and of the flavonoid pathway (chalcone synthase) in cell suspension cultures of soybean (Glycine max). After transferring cells into fresh medium, two peaks of inducibility for the enzymes by elicitor were observed, one shortly after transfer (stage I), and one at the end of the linear growth phase (stage II). Only one of the two isoenzymes of 4-coumarate: CoA ligase (“isoenzyme 2”), for which a specific involvement in flavonoid biosynthesis has been postulated, was affected by the elicitor. For two of the induced enzymes, phenylalanine ammonia-lyase and chalcone synthase, the changes in activity at stage I were shown to be preceded by large changes in their rates of synthesis, as determined by in vivo labelling with [35S] methionine and immunoprecipitation.
Similar content being viewed by others
Abbreviations
- Pmg:
-
Phytophthora megasperma f. sp. glycinea
- glyceollin:
-
is a term used to designate the 3 isomers which accumulate in challenged soybean tissue (Moesta and Grisebach 1981b)
References
Ayers AR, Ebel J, Valent B, Albersheim P (1976) Plant Physiol 57: 760–765
Betz B, Hahlbrock K (1979) FEBS Lett 107: 233–236
Börner H, Grisebach H (1982) Arch Biochem Biophys., submitted for publication
Bonner WM, Laskey RA (1974) Eur J Biochem 46: 83–88
Dische Z (1962) In: Whistler RC, Wolfram ML (eds) Methods in Carbohydrate Chemistry, vol I, Academic Press, New York, pp 478–512
Ebel J (1979) In: Luckner M, Schreiber K (eds) Regulation of Secondary Product and Plant Hormone Metabolism, Pergamon Press, Oxford, pp 155–162
Ebel J, Ayers AR, Albersheim P (1976) Plant Physiol 57: 775–779
Grisebach H, Ebel J (1978) Angew. Chem 90: 668–681, Angew. Chem Int Edit Engl 17: 635–647.
Hahlbrock K (1974) In: Street HE (ed) Tissue Culture and Plant Science 1974, Academic Press, New York, pp 363–378
Hahlbrock K (1975) Planta 125: 311–318
Hahlbrock K, Lamb CJ, Purwin C, Ebel J, Fautz E, Schäfer E (1981) Plant Physiol 67: 768–773
Keen NT (1971) Physiol Plant Pathol 1: 265–275
Knobloch K-H, Hahlbrock K (1975) Eur J Biochem 52: 311–320
Knobloch K-H, Hahlbrock K (1977) Arch Biochem Biophys 184: 237–248
Kreuzaler F, Ragg H, Heller W, Tesch R, Witt I, Hammer D, Hahlbrock K (1979) Eur J Biochem 99: 89–96
Kuan TL, Erwin DC (1980) Phytopathology 70: 333–338
Laemmli UK (1970) Nature (London) 227: 680–685
Loschke DC, Hadwiger LA, Schröder J, Hahlbrock K (1981) Plant Physiol 68: 680–685
Moesta P, Grisebach H (1981a) Arch Biochem Biophys 211: 39–43
Moesta P, Grisebach H (1981b) Arch Biochem Biophys 212: 462–467
Palmiter RD (1973) J Biol Chem 248: 2095–2106
Ragg H, Kuhn DN, Hahlbrock K (1981) J Biol Chem 256: 10061–10065
Reid MS, Bieleski RL (1968) Anal Biochem 22: 374–381
Schaffner W, Weissmann C (1973) Anal Biochem 56: 502–514
Schröder J, Betz B, Hahlbrock K (1977) Plant Physiol 60: 440–445
Schröder J, Heller W, Hahlbrock K (1979a) Plant Sci Lett 14: 281–286
Schröder J, Kreuzaler F, Schäfer E, Hahlbrock K (1979b) J Biol Chem 254: 57–65
Weber K, Pringle JR, Osborn M (1972) in: Hirs CHW, Timasheff SN (eds) Methods in Enzymology, vol 26, Academic Press, New York London, pp 3–27
Zähringer U, Ebel J, Grisebach H (1978) Arch Biochem Biophys 188: 450–455
Zähringer U, Ebel J, Mulheirn LJ, Lyne RL, Grisebach H (1979) FEBS Lett 101: 90–92
Zähringer U, Schaller E, Grisebach H (1981) Z Naturforsch 36c: 234–241
Zimmermann A, Hahlbrock K (1975) Arch Biochem Biophys 166: 54–62
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hille, A., Purwin, C. & Ebel, J. Induction of enzymes of phytoalexin synthesis in cultured soybean cells by an elicitor from Phytophthora megasperma f. sp. glycinea . Plant Cell Reports 1, 123–127 (1982). https://doi.org/10.1007/BF00272369
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272369