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Composition and molecular structure of chromoplast globules of Viola tricolor

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Abstract

Plastoglobules have been isolated in pure form from petals of the pansy, Viola tricolor L. Their chemical composition has been determined up to a recovery of 96% dry weight. Triacyl glycerols (57%) as well as carotenoids and their esters (23%) are the main constituents. Polar lipids, proteins, alkanes, phytyl esters, plastid quinones, and steryl esters have been detected in smaller amounts (cf. Table 1). The mean diameter of chromoplast globules is 280±70 nm (corresponding to a volume of 11.7×106 nm3), their buoyant density 0.93 g cm−3. The plastoglobules are devoid of a surrounding unit membrane. However, electron microscopical evidence and analytical data are consistent with a structural model envisaging the globules to consist mainly of an apolar core, covered by a ‘half unit membrane’ of polar constituents.

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Abbreviations

FA:

fatty acid

GA:

glutardialdehyde

GLC:

gas liquid chromatography

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Institut für Biologie II, Zellbiologie, Universität Freiburg, Schänzlestraße 1, D-7800, Freiburg i. Br., Federal Republic of Germany

    Paul Hansmann & Peter Sitte

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  1. Paul Hansmann
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  2. Peter Sitte
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Hansmann, P., Sitte, P. Composition and molecular structure of chromoplast globules of Viola tricolor . Plant Cell Reports 1, 111–114 (1982). https://doi.org/10.1007/BF00272366

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