Skip to main content
SpringerLink
Log in

NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA

  • Short Communication
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A new protein kinase gene [called NPKI (for nonessential protein kinase)] has been found on chromosome I of Saccharomyces cerevisiae between CDC15 and ADE1. The 435 amino acid/48 kDa gene product is very similar to known protein kinases. It is most closely related to the nimA protein of Aspergillus nidulans, displaying 45.9% identity and 63.5% similarity in the protein kinase domain. A 1.4 kb transcript of the NPKI gene was detected. Disruption of the NPKI gene impedes neither growth on glucose or a variety of other carbon sources, nor mating or sporulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Alting-Mees MA, Short JM (1989) pBluescript II: gene mapping vectors. Nucleic Acids Res 17:9494–9494

    Google Scholar 

  • Broach JR, Strathern JN, Hicks JB (1979) Transformation in yeast: Development of a hybrid cloning vector and isolation of the CANI gene. Gene 8:121–133

    Google Scholar 

  • Bullock W, Fernandez J, Short J (1987) XLl-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with betagalactosidase selection. Biotechniques 5:376–378

    Google Scholar 

  • Celenza JL, Carlson M (1986) A yeast gene that is essential for release from glucose repression encodes a protein kinase. Science 233:1175–1180

    Google Scholar 

  • Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395

    Google Scholar 

  • Elion EA, Grisafi PL, Fink GR (1990) FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation. Cell 60:649–664

    Google Scholar 

  • Hanks SK, Quinn AM, Hunter T (1988) The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains. Science 241:42–52

    Google Scholar 

  • Herman PK, Stack JH, DeModena JA, Emr SD (1991) A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole. Cell 64:425–437

    Google Scholar 

  • Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163–168

    Google Scholar 

  • Kozak M (1984) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res 12:857–872

    Google Scholar 

  • Mortimer RK, Schild D, Contopoulou CR, Kans JA (1989) Genetic map of Saccharomyces cerevisiae, Edition 10. Yeast 5:321–403

    Google Scholar 

  • Myasnikov AN, Plavnik YuA, Sasnauskas KV, Gedminene GK, Yanulaitis AA, Smirnov MN (1986) Nukleotidnaya posledovatel'nost' gena ADE1 drozhzhi Saccharomyces cerevisiae. Bioorgan Khimiya (USSR) 12:555–558

    Google Scholar 

  • Nurse P (1990) Universal control mechanism regulating onset of M-phase. Nature 344:503–508

    Google Scholar 

  • Osmani SA, Pu RT, Morris NR (1988) Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase. Cell 53:237–244

    Google Scholar 

  • Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nickler S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schweitzer B, Philippsen P (1991) CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain. Yeast 7:265–273

    Google Scholar 

  • Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sikorski RS, Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27

    Google Scholar 

  • Steensma HY, Crowley JC, Kaback DB (1987) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: Isolation and analysis of the CEN1-ADE1-CDC15 region. Mol Cell Biol 7:410–419

    Google Scholar 

  • Tabor S, Richardson CC (1987) DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci USA 84:4767–4771

    Google Scholar 

  • Teague MA, Chaleff DT, Errede B (1986) Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases. Proc Natl Acad Sci USA 83:7371–7375

    Google Scholar 

  • Toda T, Cameron S, Sass P, Zeller M, Wigler M (1987) Three different genes in S. cerevisiae encode the catalytic subunits of the CAMP-dependent protein kinase. Cell 50:277–287

    Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids: An Ml3mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

  • Zaret KS, Sherman F (1982) DNA sequence required for efficient transcription termination in yeast. Cell 28: 563–573

    Google Scholar 

Download references

Author information

Author notes

  1. Bert Schweitzer & Peter Philippsen

    Present address: Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland

Authors and Affiliations

  1. Institut für Mikro- und Molekularbiologie der Universität Gießen, Frankfurter Strasse 107, W-6300, Gießen, Germany

    Bert Schweitzer & Peter Philippsen

Authors
  1. Bert Schweitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Philippsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by C.P. Hollenberg

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schweitzer, B., Philippsen, P. NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA . Molec. Gen. Genet. 234, 164–167 (1992). https://doi.org/10.1007/BF00272358

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272358

Key words

Search

Navigation