Summary
Retention or loss of the two new mitochondrial antimycin A resistance loci AI and AII has been analyzed in a large number of stable cytoplasmic petite mutants. Using these deletion mutants it was possible to localize the two antimycin A resistance loci in the OI-OII region of mitochondrial DNA. The genetic loci are mapped in the following order: OII-AI-AII-cobl-OI. The mapping relationship of mutants resistant to antimycin A or funiculosin to various cob mutants is described.
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Burger, G., Lang, B., Bandlow, W., Schweyen, R.J., Backhaus, B., Kaudewitz, F.: Antimycin resistance in Saccharomyces cerevisiae: a new mutation on the mitDNA conferring antimycin resistance on the mitochondrial respiratory chain. Biochem. biophys. Res. Commun. 72, 1201–1208 (1976)
Colson, A.-M., The Van L., Convent, B., Briquet, M., Goffeau, A.: Mitochondrial heredity of resistance to 3-(3,4-dichlorphenyl)-1,1-dimethylurea, an inhibitor of cytochrome b oxidation, in Saccharomyces cerevisiae. Europ. J. Biochem. 74, 521–526 (1977)
Foury, F., Tzagoloff, A.: Assembly of the mitochondrial membrane system XIX. Genetic characterization of mit- mutants with deficiencies in cytochrome oxidase and coenzyme QH2-cytochrome c reductase. Molec. gen. Genet. 149, 43–50 (1976)
Groot Obbink, D.J., Hall, R.M., Linnane, A.W., Lukins, H.B., Monk, B.C., Spithill, T.W., Trembath, M.K.: Mitochondrial genes involved in the determination of mitochondrial membrane proteins. In: (C. Saccone, A.M. Kroon, eds.), The genetic function of mitochondrial DNA, pp. 163–173. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Groot Obbink, D.J., Spithill, T.W., Maxwell, R.J., Linnane, A.W.: Biogenesis of mitochondria 48. Mikamycin resistance in Saccharomyces cerevisiae — A mitochondrial mutation conferring resistance to an antimycin A-like contaminant in mikamycin. Molec. gen. Genet. 151, 127–136 (1977)
Jakob, H.: Technique de synchronisation de la formation des zygotes chez la levure Saccharomyces cerevisiae. C.R. Acad. Sci. (Paris) 254, 3909 (1962)
Katan, M.B., Van Harten-Loosbroek, N., Groot, G.S.P.: The cytochrome bc1 complex of yeast mitochondria. Site of translation of the polypeptides in vivo. Europ. J. Biochem. 70, 409–417 (1976)
Kotylak, Z., Slonimski, P.P.: Joint control of cytochromes a and b by a unique mitochondrial DNA region comprising four genetic loci. In: (C. Saccone, A.M. Kroon, eds.), The genetic function of mitochondrial DNA, pp. 143–154. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Michaelis, G.: Cytoplasmic inheritance of antimycin A resistance in Saccharomyces cerevisiae. Molec. gen. Genet. 146, 133–137 (1976)
Michaelis, G., Douglass, S., Tsai, M.-J., Criddle, R.S.: Mitochondrial DNA and suppressiveness of petite mutants in Saccharomyces cerevisiae. Biochem. Genet. 5, 487–495 (1971)
Pajot, P., Wambier-Kluppel, M.L., Kotylak, Z., Slonimski, P.P.: Regulation of cytochrome oxidase formation by mutations in a mitochondrial gene for cytochrome b. In: (Th. Bücher, W. Neupert, W. Sebald, S. Werner, eds.), Genetics and biogenesis of chloroplasts and mitochondria, pp. 443–451. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Pratje, E., Michaelis, G.: Two mitochondrial antimycin A resistance loci in Saccharomyces cerevisiae. In: (Th. Bücher, W. Neupert, W. Sebald, S. Werner, eds.), Genetics and biogenesis of chloroplasts and mitochondria, pp. 443–451. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Pratje, E., Michaelis, G.: Allelism studies of mitochondrial mutants resistant to antimycin A or funiculosin in Saccharomyces cerevisiae. Molec. gen. Genet. 152, 167–174 (1977)
Rieske, J.S.: Composition, structure, and function of complex III of the respiratory chain. Biochim. biophys. Acta (Amst.) 456, 195–247 (1976)
Slonimski, P.P., Tzagoloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Europ. J. Biochem. 61, 27–41 (1976)
Subik, J.: Mucidin-resistant antimycin A-sensitive mitochondrial mutant of Saccharomyces cerevisiae. FEBS Letters 59, 273–276 (1975)
Subik, J., Kovacova, V., Takacsova, G.: Mucidin resistance in yeast. Isolation, characterization and genetic analysis of nuclear and mitochondrial mucidin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 73, 275–286 (1977)
Tzagoloff, A., Akai, A., Needleman, R.B.: Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase. Proc. nat. Acad. Sci. (Wash.) 72, 2054–2057 (1975)
Tzagoloff, A., Foury, F., Akai, A.: Assembly of the mitochondrial membrane system XVIII. Genetic loci on mitochondrial DNA involved in cytochrome b biosynthesis. Molec. gen. Genet. 149, 33–42 (1976)
Weiss, H.: Subunit composition and biogenesis of mitochondrial cytochrome b. Biochim. biophys. Acta (Amst.) 456, 291–313 (1976)
Weiss, H., Ziganke, B.: Cytochrome b in Neurospora crassa mitochondria. Site of translation of the heme protein. Europ. J. Biochem. 41, 63–71 (1974)
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Communicated by W. Gajewski
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Michaelis, G., Pratje, E. Mapping of the two mitochondrial antimycin a resistance loci in Saccharomyces cerevisiae . Molec. Gen. Genet. 156, 79–85 (1977). https://doi.org/10.1007/BF00272255
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DOI: https://doi.org/10.1007/BF00272255