Skip to main content
SpringerLink
Log in

Mapping of the genes controlling excision repair of Pyrimidine photoproducts in Bacillus subtilis

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

Twenty nine uvr mutations of Bacillus subtilis Marburg have been classified into two groups by mutual transformation crosses. One, uvrA, contained 20 mutations and involved uvrAl which had been isolated by Reiter and Strauss (1965) and linked to the hisA gene by Hoch and Anagnostopoulos (1970). Another group, uvrB, contained 9 mutations and the linkage relationship has been established as argA-polA-uvrB-leu-pheA. Both of them seemed to govern the incision reaction upon DNA containing pyrimidine dimers or spore photoproducts. It thus seems that in B. subtilis, as in Escherichia coli, the process of excision repair is controlled by the products of two or more genes not closely linked in the chromosome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bishop, O.E., Brown, L.R.: Ethidium bromide-resistant mutant of Bacillus subtilis. J. Bact. 115, 1077–1083 (1973)

    Google Scholar 

  • Carlton, B.C.: Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J. Bact. 91, 1795–1803 (1966)

    Google Scholar 

  • Cleaver, J.E., Bootsma, D.: Xeroderma pigmentosum. Biochemical and genetic characteristics. Annu. Rev. Genet. 9, 18–38 (1975)

    Google Scholar 

  • Dubnau, D.: Genetic mapping of Bacillus subtilis. In: Methods in enzymology (Grossman, L., Moldave, K., eds.), Vol. 21, pp. 430–438. New York and London: Academic Press 1971

    Google Scholar 

  • Ephrati-Elizur, E., Srinivasan, P.R., Zamenof, S.: Genetic analysis, by means of transformation, of histidine linkage groups in Bacillus subtilis. Proc. nat. Acad. Sci. (Wash.) 47, 56–63 (1961)

    Google Scholar 

  • Farmer, J.L., Rothman, F.: Transformable thymine-requiring mutant of Bacillus subtilis. J. Bact. 89, 262–263 (1965)

    Google Scholar 

  • Game, J.C., Cox, B.S.: Epistatic interaction between four rad loci in yeast. Mutation Res. 16, 353–362 (1972)

    Google Scholar 

  • Grossman, L.: Enzymes involved in the repair of DNA. Advanc. Radiat. Biol. 4, 77–129 (1974)

    Google Scholar 

  • Hill, T., Prakash, L., Strauss, B.: Mutagen stability of alkylationsensitive mutants of Bacillus subtilis. J. Bact. 110, 47–55 (1975)

    Google Scholar 

  • Hoch, J.A., Anagnostopoulos, C.: Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J. Bact. 103, 295–301 (1970)

    Google Scholar 

  • Howard-Flanders, P., Boyce, R.P., Theriot, L.: Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53, 1119–1136 (1966)

    Google Scholar 

  • Munakata, N.: Genetic analysis of a mutant of Bacillus subtilis producing ultraviolet-sensitive spores. Molec. gen. Genet. 104, 258–263 (1969)

    Google Scholar 

  • Munakata, N.: Repair of ultraviolet-induced damage in transforming DNA of Bacillus subtilis. Japan. J. Genet. 45, 1–9 (1970)

    Google Scholar 

  • Munakata, N., Ikeda, Y.: A mutant of Bacillus subtilis producing ultraviolet-sensitive spores. Biochem. biophys. Res. Commun. 33, 469–475 (1968)

    Google Scholar 

  • Munakata, N., Ikeda, Y.: Inactivation of transforming DNA by ultraviolet irradiation; a study with ultraviolet-sensitive mutants of Bacillus subtilis. Mutation Res. 7, 133–139 (1969)

    Google Scholar 

  • Munakata, N., Rupert, S.: Dark repair of DNA containing “spore photoproduct” in Bacillus subtilis. Molec. gen. Genet. 130, 239–250 (1974)

    Google Scholar 

  • Munakata, N., Rupert, S.: Effects of DNA-polymerase-defective and recombination-deficient mutations on the ultraviolet sensitivity of Bacillus subtilis spores. Mutation Res. 27, 157–169 (1975)

    Google Scholar 

  • Okubo, S., Romig, W.R.: Comparison of ultraviolet sensitivity of Bacillus subtilis bacteriophage SPO2 and its infections DNA. J. molec. Biol. 14, 130–142 (1965)

    Google Scholar 

  • Reiter, H., Strauss, B.: Repair of damage induced by a monofunctional alkylating agent in a transformable ultraviolet-sensitive strain of Bacillus subtilis. J. molec. Biol. 14, 179–194 (1965)

    Google Scholar 

  • Shibata, T., Saito, H.: Repair of ultraviolet-induced DNA damage in the subcellular system of Bacillus subtilis. Mutation Res. 20, 159–173 (1973)

    Google Scholar 

  • Spizizen, J.: Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. nat. Acad. Sci. (Wash.) 44, 1072–1078 (1958)

    Google Scholar 

  • Takahashi, H., Ikeda, Y.: The intramolecular heterogeneity of Bacillus subtilis DNA studies by isopycnic CsCl gradient. Biochim. biophys. Acta (Amst.) 213, 523–525 (1970)

    Google Scholar 

  • Tanooka, H., Sakakibara, Y.: Radioresistant nature of the transforming activity of DNA in bacterial spores. Biochim. biophys. Acta (Amst.) 155, 130–142 (1968)

    Google Scholar 

  • Yasuda, S., Sekiguchi, M.: T4 endonuclease involved in repair of DNA. Proc. nat. Acad. Sci. (Wash.) 67, 1839–1845 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Radiobiology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, 104, Tokyo, Japan

    Nobuo Munakata

Authors
  1. Nobuo Munakata
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by B.A. Bridges

Rights and permissions

Reprints and permissions

About this article

Cite this article

Munakata, N. Mapping of the genes controlling excision repair of Pyrimidine photoproducts in Bacillus subtilis . Molec. Gen. Genet. 156, 49–54 (1977). https://doi.org/10.1007/BF00272251

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272251

Keywords

Search

Navigation