Summary
Meiotic recombination among unselected tetrads was analyzed genetically and physically in a heterologous cross where one parent carried six copies of a 1.6 kb CUP1 repeat while the other parent carried seven copies of a 1.1 kb repeat. In the heterologous cross, 140 unselected, complete tetrads were subjected to Southern analysis and 20% exhibited meiotic copy number alterations at the CUP1 locus. Most events, more than 75%, involved only a single spore of a tetrad, and were largely intrachromosomal or sister chromatid events. However, some conversions and associated cross-overs between homologs were also observed. We propose that the high level of heterologies interferes with homologous exchanges and leads to an increase in intrachromosomal events.
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Borts RH, Haber JE (1987) Meiotic recombination in yeast: alteration by multiple heterozygosities. Science 237:1459–1465
Fink GR, Petes TD (1984) Gene conversion in the absence of recombination. Nature 310:728–729
Fogel S, Hurst DD (1967) Meiotic gene conversion in yeast tetrads and the theory of recombination. Genetics 57:455–481
Fogel S, Welch J (1982) Tandem gene amplification mediates copper resistance in yeast. Proc Natl Acad Sci USA 79:5342–5346
Fogel S, Mortimer RK, Lusnak K, Tavares F (1979) Meiotic gene conversion — a signal of the basic recombination event in yeast. Cold Spring Harbor Symp Quant Biol 43:1325–1341
Fogel S, Mortimer RK, Lusnak K (1981) Mechanisms of meiotic gene conversion, or “Wanderings on a foreign strand”. In: Strathern JN et al. (eds) The molecular biology of the yeast Saccharomyces, vol 1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 289–339
Fogel S, Welch J, Louis E (1984) Meiotic gene conversion mediates gene amplification in yeast. Cold Spring Harbor Symp Quant Biol 49:55–65
Hurst DD, Fogel S, Mortimer RK (1972) Conversion-associated recombination in yeast. Proc Natl Acad Sci USA 61:101–105
Jackson J, Fink GR (1985) Meiotic recombination between duplicated genetic elements in Saccharomyces cerevisiae. Genetics 109:303–332
Judd SR, Petes TD (1988) Physical lengths of meiotic and mitotic gene conversion tracts in Saccharomyces cerevisiae. Genetics 118:401–410
Klein HL (1984) Lack of association between intrachromosomal gene conversion and reciprocal exchange. Nature 310:748–753
Lichten M, Borts RH, Haber JE (1987) Meiotic gene conversion and crossing-over between dispersed homologous sequences occurs frequently in Saccharomyces cerevisiae. Genetics 115:233–246
Maeda N, Smithies O (1986) The evolution of multigene families: human haptoglobin genes. Annu Rev Genet 20:81–108
Maloney DH, Fogel S (1987) Gene conversion, unequal crossing-over and mispairing at a non-tandem duplication during meiosis of Saccharomyces cerevisiae. Curr Genet 12:1–7
Mitchell MB (1955) Aberrant recombination of pyroxidine mutants of Neurospora. Proc Natl Acad Sci USA 41:215–220
Mortimer RK, Fogel S (1974) Genetical interference and gene conversion. In: Grell RF (ed) Mechanisms in recombination. Plenum, New York, pp 263–275
Nathans J, Plantanida TP, Eddy RL, Shows TB, Hogness DS (1986a) Molecular genetics of inherited variation in human color vision. Science 232:203–210
Nathans J, Thomas D, Hogness DS (1986b) Molecular genetics of human color vision: the genes encoding blue, green and red pigments. Science 232:193–202
Nathans J, Davenport CM, Maumenee IH, Lewis RA, Hejtmancik JF, Litt M, Lovrien E, Weleber R, Bachynski B, Zwas F, Klingaman R, Fishman G (1989) Molecular genetics of human blue cone monochromacy. Science 245:831–838
Petes TD (1980) Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell 19:765–774
Symington LS, Petes TD (1988) Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III. Mol Cell Biol 8:595–604
Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW (1983) The double strand break model for recombination. Cell 33:25–35
Taub RA, Hollis GF, Hieter PA, Korsmeyer S, Waldmann TA, Leder P (1983) Variable amplification of immunoglobulin lightchain genes in human populations. Nature 304:172–174
Weiss EH, Mellor A, Golden L, Fahrner K, Simpson E, Hurst J, Flavell RA (1983) The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events. Nature 301:671–674
Welch JW, Fogel S, Cathala, Karin M (1983) Industrial yeasts display tandem gene iteration at the CUP1 region. Mol Cell Biol 3:1353–1361
Welch JW, Maloney DH, Fogel S (1990) Unequal crossing-over and gene conversion at the amplified CUP1 locus of yeast. Mol Gen Genet 222:304–310
Wheeler CJ, Maloney D, Fogel S, Goodenow RS (1990) Microconversion between murine H-2 genes integrated into yeast. Nature 347:192–194
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Welch, J.W., Maloney, D.H. & Fogel, S. Gene conversions within the Cup1 r region from heterologous crosses in Saccharomyces cerevisiae . Molec. Gen. Genet. 229, 261–266 (1991). https://doi.org/10.1007/BF00272164
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DOI: https://doi.org/10.1007/BF00272164