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Hyphal growth of Phytophtora cinnamomi on pine callus tissue

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Abstract

A procedure was developed which demonstrates the expression of differential resistance in pine callus tissues to the fungal pathogen Phytophthora cinnamomi Rands. Callus tissues were maintained on a modified Murashige and Skoog medium with 10−5M 2,4-D and inoculated with hyphae of P. cinnamomi at 26°C in the dark. The number of intracellular hyphae was used as an index of resistance. Loblolly and loblolly × shortleaf pine hybrids were determined to be more resistant to infection and invasion by the fungus than were shortleaf and Virginia pine.

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Abbreviations

(AL):

loblolly pine—Alabama

(PL):

South Carolina

(AS):

shortleaf pine—Alabama

(CS):

Georgia

(AV):

Virginia pine—Alabama

(H1):

loblolly × shortleaf pine hybrids—14–42 × 6-I-43

(H2):

I-523 × 6-D-8

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Communicated by I. K. Vasil

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Jang, J.C., Tainter, F.H. Hyphal growth of Phytophtora cinnamomi on pine callus tissue. Plant Cell Reports 8, 741–744 (1990). https://doi.org/10.1007/BF00272108

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  • DOI: https://doi.org/10.1007/BF00272108

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