Abstract
To develop an adventitious regeneration system for pear cultivars, several experiments were conducted with 2 cultivars of Pyrus communis L. (‘Seckel’ and ‘Louise Bonne’) and one cultivar of P. bretschneideri Rehd. (‘Crystal Pear’). Half-leaves, taken from shoots proliferating on Lepoivre medium, were plated in petri-dishes on medium supplemented with various combinations of cytokinins and auxins. Cultures of the above cultivars had been established from mature trees. Among the growth regulators tested, thidiazuron (TDZ), combined with naphthalene-acetic acid (NAA), was the most efficient for stimulation of adventitious shoots. The optimum level of TDZ was about 3 uM; shoot regeneration was observed over a wide range of TDZ and NAA concentrations (0.5 to 5 uM and 2.5 to 13 um, respectively). Among different macronutrient compositions, 1/2 and 1/4 Murashige and Skoog were the most effective. Sucrose concentrations (10 to 50 g L-1) had a linear significant effect on shoot regeneration of ‘Crystal Pear’.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
- NAA:
-
a-naphthaleneacetic acid
- NoA:
-
2-naphthoxyacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- TDZ:
-
thidiazuron (N-phenyl-N'-1,2,3-thidiazol-5-ylurea)
- MS:
-
Murashige and Skoog (1962) macroelements
- L:
-
Lepoivre (Quoirin and Lepoivre, 1977) macroelements
References
Druart P (1980) Sci Hort 12:339–342
Evaldsson I (1985) Swedish J Agric Res 15:119–122
Hutchinson JF, Zimmerman RH (1987) Hort Review 273–349
James DJ, Mackenzie KAD, Mahlotra SB (1987) Theor Appl Genet 73:589–594
James DJ, Passey AJ, Malhotra SB (1984) Plant Cell Tissue Organ Culture 3(4):333–341
Jones OP, Gayner JA, Watkins R (1984) J Hort Sci 59(4):463–467
Kerns HR, Meyer MM (1986) HortSci 21(5):1209–1210
Mehra PN, Jaidka K (1979) Phytomorphology 29:286–298
Mehra PN, Jaidka K (1985) Phytomorphology 35:1–9
Murashige T, Skoog F (1962) Physiologia Pl 15:473–497
Ochatt SJ, Caso OH (1986) J Plant Physiol 122:243–249
Quoirin M, Lepoivre P (1977) Acta Hortic 78:437
Revilla MA, Ochatt SJ, Doughty S, Power JB (1987) Plant Science, 50:133–137.
Singha S (1986) Pear (Pyrus communis). In YPS Bajaj (ed.) Biotechnology in Agriculture and Forestry Vol. 1:Trees, Springer-Verlag Berlin, pp 198–206
Steel RGD, Torrie J (1980) Principles and procedures of statistics. McGraw-Hill Book Company, pp 177–181
van Nieuwkerk JP, Zimmerman RH, Fordham I (1986) HortScience 21(3):516–518
Wanas WH, Callow JA, Withers (1986) In Withers LA and Alderson PG (eds.), Plant tissue culture and its agricultural applications, Butter-worths, London, pp 285–290
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Communicated by J.M. Widholm
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Chevreau, E., Skirvin, R.M., Abu-Qaoud, H.A. et al. Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro. Plant Cell Reports 7, 688–691 (1989). https://doi.org/10.1007/BF00272062
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DOI: https://doi.org/10.1007/BF00272062