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In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L.

Plant Cell Reports volume 7, pages 626–627 (1989)Cite this article

Abstract

Five different genotypes from in vitro as well as greenhouse grown melon plants were shown to be highly responsive for in vitro shoot formation from leaf explants when placed on basic MS medium supplemented with 1 mg/l 6-benzylaminopurine. In addition, a very suitable regeneration system was obtained when cotyledon pieces of mature seeds were incubated on the same culture medium. In this case, the first shoots already appeared after 10 days of incubation, and hundreds of shoots were formed on the cut surface 3 to 4 weeks later. Explants from mature cotyledons derived from seedlings did not lead to any shoot formation.

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Abbreviations

MS:

Murashige and Skoog

IAA:

3-indoleacetic acid

BA:

6-benzylaminopurine

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Affiliations

  1. Nunhems Zaden BV, Voort 6, 6083 AC, Nunhem, The Netherlands

    Rob Dirks & Marjan van Buggenum

Authors
  1. Rob Dirks
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  2. Marjan van Buggenum
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Communicated by N. Amrhein

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Dirks, R., van Buggenum, M. In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L.. Plant Cell Reports 7, 626–627 (1989). https://doi.org/10.1007/BF00272045

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  • DOI: https://doi.org/10.1007/BF00272045

Keywords

  • Regeneration System
  • Plant Regeneration
  • Melon
  • Mature Seed
  • Leaf Explants