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In vivo transfer of the Ti-plasmid of Agrobacterium tumefaciens to Escherichia coli

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Summary

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crowngall tumours on plants.

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Authors and Affiliations

  1. Laboratorium voor Genetika en voor Histologie en Genetika, Rijksuniversiteit Gent, Ledeganckstraat 35, B-9000, Gent, Belgium

    M. Holsters, B. Silva, F. Van Vliet, J. P. Hernalsteens, C. Genetello, M. Van Montagu & J. Schell

Authors
  1. M. Holsters
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  2. B. Silva
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  3. F. Van Vliet
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  4. J. P. Hernalsteens
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  5. C. Genetello
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  6. M. Van Montagu
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  7. J. Schell
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Additional information

Communicated by G. Melchers

This work was supported by grants from the Kankerfonds van de A.S.L.K. and from the Fonds voor Kollektief Fundamenteel Onderzoek (n0 10.316) to J.S. and M.V.M.

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Holsters, M., Silva, B., Van Vliet, F. et al. In vivo transfer of the Ti-plasmid of Agrobacterium tumefaciens to Escherichia coli . Molec. Gen. Genet. 163, 335–338 (1978). https://doi.org/10.1007/BF00271963

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  • DOI: https://doi.org/10.1007/BF00271963

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