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Repetitive DNA in Escherichia coli: Multiple sequences complementary to small stable RNAs

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Summary

Radioactively labeled 4.5S, 6S, and 10S RNAs from Escherichia coli were hybridized to EcoRI fragments from the E. coli genome. Each of these molecules bound to more than one DNA fragment. Cot curve analysis of the kinetics of the annealing of these RNAs to denatured E. coli DNA suggests that the DNA corresponding to each of these molecules is reiterated in the genome. These experiments also suggest that these reiterated sequences are non adjacent.

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References

  • Apirion, D., Lassar, A.B.: A conditional lethal mutant of Escherichia coli which affects the processing of ribosomal RNA. J. Biol. Chem. 253, 1738–1742 (1978)

    Google Scholar 

  • Bishop, J.O.: Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid. Biochem. J. 126, 171–185 (1972)

    Google Scholar 

  • Bothwell, A.L.M., Garber, R.L., Altman, S.: Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5S RNA. J. Biol. Chem. 251, 7709–7716 (1976)

    Google Scholar 

  • Britten, R., Kohne, D.:Repeated sequences in DNA. Science 161, 529–540 (1968)

    Google Scholar 

  • Brownlee, G.G.: Sequence of 6S RNA of E. coli. Nature New Biol. 229, 147–149 (1971)

    Google Scholar 

  • Engberg, J., Hellung-Larsen, P., Frederiksen, S.: Isolation and DNA-RNA hybridization properties of small molecular weight nuclear RNA components from baby-hamster-kidney cells. Eur. J. Biochem. 41, 321–328 (1974)

    Google Scholar 

  • Fournier, M.J., Webb, E.: Escherichia coli 4.5S and 6S RNAs are derived from reiterated sequences of DNA. Fed. Proc. 36, 659 (1977)

    Google Scholar 

  • Gegenheimer, P., Watson, N., Apirion, D.: Multiple pathways for primary processing of ribosomal RNA in Escherichia coli. J. Biol. Chem. 252, 3064–3073 (1977)

    Google Scholar 

  • Ghora, B.K., Apirion, D.: 5S ribosomal RNA is contained within a 25S ribosomal RNA that accumulates in mutants of Escherichia coli defective in processing of ribosomal RNA. J. Mol. Biol. 127, 507–513 (1979)

    Google Scholar 

  • Griffin, B.E., Baillie, D.L.: Precursors of stable RNA accumulated in a mutant of E. coli. FEBS Lett. 34, 273–279 (1973)

    Google Scholar 

  • Hellung-Larsen, P., Frederiksen, S.: Occurrence and properties of low molecular weight RNA components from cells at different taxonomic levels. Comp. Biochem. Physiol. 58B, 273–281 (1977)

    Google Scholar 

  • Kenerley, M.E., Morgan, E.A., Post, L., Lindahl, L., Nomura, M.: Characterization of hybrid plasmids carrying individual ribosomal RNA transcription units of Escherichia coli. J. Bacteriol. 132, 931–949 (1977)

    Google Scholar 

  • Kim, K., Apirion, D., Ghora, B.: Small stable RNA of Neurospora crassa. Mol. Gen. Genet. 160, 25–32 (1978)

    Google Scholar 

  • Kiss, A., Sain, B., Venetianer, P.: The number of rRNA genes in Escherichia coli. FEBS Lett. 79, 77–79 (1977)

    Google Scholar 

  • Lee, S., Bailey, S., Apirion, D.: Small stable RNAs from Escherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle conatining 6S RNA. J. Bacteriol. 133, 1015–1023 (1978)

    Google Scholar 

  • Marzluff, W.F., White, E.L., Benjamin, R., Huang, R.C.: Low molecular weight RNA species from chromatin. Biochemistry 14, 3715–3724 (1975)

    Google Scholar 

  • Melli, M., Whitfield, C., Rao, K.V., Richardson, M., Bishop, J.O.: DNA-RNA hybridization in vast DNA excess. Nature New Biol. 231, 8–12 (1971)

    Google Scholar 

  • Miura, K.I.: Preparation of bacterial DNA by the phenol-pH 9-RNase method. Methods in enzymology, Vol. XII A, pp. 543–545. L. Grossman and K. Moldave (eds.) New York: Academic Press 1967

    Google Scholar 

  • Monahan, J.: The structure origin and function(s) of RNA in the nuclei of eukaryotic cells. Int. Rev. Cytol. in press.

  • Pace, N.R.: Structure and synthesis of the ribosomal RNA of prokaryotes. Bacteriol. Rev. 37, 562–603 (1973)

    Google Scholar 

  • Raj, N.B.K., Ro-Choi, T.S., Busch, H.: Nuclear ribonucleoprotein complexes containing U1 and U2 RNA. Biochemistry 14, 4380–4385 (1975)

    Google Scholar 

  • Southern, E.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)

    Google Scholar 

  • Weinberg, R.A., Penman, S.: Small molecular weight monodisperse nuclear RNA. J. Mol. Biol. 38, 289–304 (1968)

    Google Scholar 

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Author notes

  1. Stephen C. Bailey

    Present address: Department of Enzyme Research and Development, Sigma Chemical Company, 3500 DeKalb Street, 63118, St. Louis, Missouri, U.S.A.

Authors and Affiliations

  1. Department of Microbiologiy and Immunology, Division of Biology and Biomedical Sciences, Washington University, School of Medicine, 63110, St. Louis, Missouri, USA

    Stephen C. Bailey & David Apirion

Authors
  1. Stephen C. Bailey
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  2. David Apirion
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Communicated by H.G. Wittmann

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Bailey, S.C., Apirion, D. Repetitive DNA in Escherichia coli: Multiple sequences complementary to small stable RNAs. Molec. Gen. Genet. 172, 339–343 (1979). https://doi.org/10.1007/BF00271734

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  • DOI: https://doi.org/10.1007/BF00271734

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