Summary
The 5′ flanking region of a glutelin gene was analyzed for interactions with nuclear proteins from immature rice seed. The specific region between positions-272 and -99 was shown to interact with nuclear proteins from immature seeds, but not with those of leaves and roots. Methylation interference experiments revealed that one factor interacted with a specific sequence element between positions -130 and -120 relative to the transcriptional start site. The sequence specificity of this DNA-protein interaction was confirmed by competition experiments using synthetic oligonucleotides. By using a synthetic oligonucleotide as a probe it was also shown that the binding activity was closely correlated with the mRNA levels of this gene during seed maturation.
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Communicated by J. Schell
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Takaiwa, F., Oono, K. Interaction of an immature seed-specific trans-acting factor with the 5′ upstream region of a rice glutelin gene. Molec. Gen. Genet. 224, 289–293 (1990). https://doi.org/10.1007/BF00271563
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DOI: https://doi.org/10.1007/BF00271563