Abstract
The RNA of American hop latent virus (AHLV) has a molecular size of 7.7 kb measured in agarose gels. Translation products of AHLV RNA in rabbit reticulocyte and wheat-germ cell-free systems ranged in size up to 200 kD. Time-course experiments indicated that a 36 kD peptide, immunoprecipitated by antivirion sera, was synthesized early followed by the sequential appearance of peptides of increasing Mr. An excess of the amino-acid analogue L-canavanine for arginine specifically reduced the quantity of the 36 kD peptide and induced synthesis of a 38-kD peptide in both rabbit reticulocyte and wheat germ. Translation products were not altered by the addition of a reducing agent, and no product appeared to be produced due to limitation of tRNA species or by readthrough. Synthesis of AHLV RNA-directed peptides was blocked by the cap analogue m7G5′ ppp5′G, suggesting the presence of a cap structure at the 5′ terminus.
Similar content being viewed by others
References
AdamsA.N. and BarbaraD.J., Ann Appl Biol 101, 483–494, 1982.
Wetter C. and Milne R.G., in Kurstak E. (ed.) Handbook of Plant Virus Infections Comparative Diagnosis. Elsevier/North Holland Biomedical Press, 1981, pp. 695–730.
Adams A.N. and Barbara D.J., Ann Appl Biol 101, 495–500.
FosterG.D. and MillsP.R., Potato Res 33, 487–495, 1990.
ManiatisT., FritschE.F. and SambrookJ., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982.
LaemmliU.K., Nature (London) 227, 680–685, 1970.
MayoM.A. and ReddyD.V.R., J Gen Virol 66, 1347–1351, 1985.
AhlquistP., DasguptaR. and KaesbergP., J Mol Biol 172, 369–383, 1984.
MackenzieD.J., TremaineJ.H. and Stace-SmithR., J Gen Virol 70, 1053–1063, 1989.
RupasovV.V., Morozov S.Yu, KanyukaK.V., et al., J Gen Virol 70, 1861–1869, 1989.
DasguptaR., HaradaF. and KaesbergP., J Virol 18, 260–267, 1976.
MayoM.A., BarkerH. and HarrisonB.D., J Gen Virol 59, 149–162, 1982.
KoenigI. and FritschC., J Gen Virol 60, 343–353, 1982.
SonenbergN., ShatkinA.J., RicciardiR.P., et al., Nucleic Acids Res 5, 2501–2512, 1978.
DaviesJ.W. and HullR., J Gen Virol 61, 1–14, 1982.
GuilfordP.J. and ForsterR.L.S., J Gen Virol 67, 83–90, 1986.
DoljaV.V., GramaD.P., Morozov S.Yu et al., FEBS Lett 214, 308–312, 1987.
ForsterR.L.S., GuilfordP.J. and FauldsD.V., J Gen Virol 68, 181–190, 1987.
DoughertyW.G. and HiebertE., Virology 104, 183–194, 1980.
BendenaW.G., AbouhaidarM. and MackieG.A., Virology 140, 257–268, 1985.
PelhamH.R.B., Eur J Biochem 105, 445–451, 1980.
PelhamH.R.B., Virology 96, 463–477, 1979.
VanceV.B. and BeachyR.N., Virology 132, 271–281, 1984.
MurphyE.C. and ArlinghausR.B., J Virol 33, 954–961, 1980.
RedmanC.M., AvellinoG. and YuS., J Biol Chem 258, 3446–3452, 1983.
YehS.D. and GonsalvesD., Virology 143, 260–271, 1985.
HarbisonS.A., DaviesJ.W. and WilsonT.M.A., J Gen Virol 66, 2597–2604, 1985.
MorchM.D. and BenicourtC., Eur J Biochem 105, 445–451, 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Foster, G.D., Mills, P.R. Cell-free translation of American hop latent virus RNA. Virus Genes 5, 327–334 (1991). https://doi.org/10.1007/BF00271531
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00271531