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The isolation and genetic characteristics of lambda transducing phages of the uvrA + and uvrC + genes of E. coli K12

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Summary

Lambda transducing phages carying the excision repair genes uvrA + and uvrC + were selected from a pool of λ phages carrying EcoR1 fragments of E. coli DNA. These phages and also λuvrB + (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA -, uvrB - or uvrC -. Lambda uvrA + was found to transduce strains carrying uvrA - but not those carrying uvrB - or uvrC -, to normal ultraviolet resistance. Similarly, λuvrB + and λuvrC + were found to complement only the corresponding uvr - allele. The λ transducing phages were co-transduced with gal + by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site.

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Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, School of Medicine, 06520, New Haven, Connecticut, USA

    Jeffrey Auerbach & Paul Howard-Flanders

  2. Department of Therapeutic Radiology, Yale University, School of Mediine, 06520, New Haven, Connecticut, USA

    Jeffrey Auerbach & Paul Howard-Flanders

Authors
  1. Jeffrey Auerbach
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  2. Paul Howard-Flanders
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Communicated by H.W. Boyer

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Auerbach, J., Howard-Flanders, P. The isolation and genetic characteristics of lambda transducing phages of the uvrA + and uvrC + genes of E. coli K12. Molec. Gen. Genet. 168, 341–344 (1979). https://doi.org/10.1007/BF00271506

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  • DOI: https://doi.org/10.1007/BF00271506

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