Summary
We have cloned the entire r-determinant of the antibiotic resistance plasmid R100.1 on the plasmid vectors pCR1 and pSC201. We find that the hybrid plasmids segregate from cultures in which replication of the vector is blocked. This suggests that the r-det is not capable of autonomous replication.
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References
Cabello, F., Timmis, K., Cohen, S.: Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 259, 285–290 (1976)
Chandler, M., Allet, B., Gallay, E., Boy de la Tour, E., Caro, L.: Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R 100.1. Mol. Gen. Genet. 153, 289–295 (1977a)
Chandler, M., Silver, L., Frey, J., Caro, L.: Suppression of an Escherichia coli dnaA mutation by the integrated R factor R 100.1: II Genration of small plasmids after integration. J. Bacteriol. 130, 301–303 (1977b)
Chandler, M., Silver, L., Lane, D., Caro, L.: Cold Spring Harbor Symp. Quant. Biol. XLIII (in press). Properties of an autonomous r-determinant from R 100.1 (1978)
Cohen, S.N., Chang, A.C.Y., Hsu, L.: Non-chromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)
Cohen, S.N., Miller, C.: Multiple molecular species of circular R factor DNA isolated from Escherichia coli. Nature 224, 1273–1277 (1969)
Cohen, S.N., Miller, C.: Non-chromosomal antibiotic resistance in bacteria. III Isolation of the discrete transfer unit of the R factor R1. Proc. Natl. Acad. Sci. U.S.A. 67, 510–516 (1970)
Covey, C., Richardson, D., Carbon, J.: A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid. Mol. Gen. Genet. 145, 155–158 (1976)
Grindley, N., Kelley, W.: Effects of different alleles of the E. coliK 12 polA gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143, 311–318 (1976)
Kretschmer, P.J., Cang, A., Cohen, S.N.: Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J. Bacteriol. 124, 225–231 (1975)
Lane, D., Chandler, M.: Mapping of the drug resistance genes carried by the r-determinant of the T 100.1 plasmid. Mol. Gen. Genet. 157, 17–23 (1977)
Mattson, T., Van Houwe, G., Bolle, A., Epstein, R.H.: Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mol. Gen. Genet. 154, 319–326 (1977)
Monk, M., Kinross, J.: Conditional lethality of recA and recB derivated of a strain of E. coliK 12 with a temperature sensitive deoxyribonucleic acid polymerase I. J. Bacteriol. 109, 971–978 (1972)
Nisioka, T., Mitani, M., Clowes, R.: Molecular recombination between R factor deoxyribonucleic acid molecules in Escherichia coli host cells. J. Bacteriol. 130, 166–177 (1970)
Rownd, R., Mickel, S.: Dissociation and reassociation of RTF and r-determinants of the R factor NR1 in Proteus mirabilis. Nature 234, 40–43 (1971)
Sharp, P.A., Cohen, S.N., Davidson, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of E. coli. II. Structure of drug resistance (R) factors and F factors. J. Mol. Biol. 75, 235–255 (1973)
Watanabe, T., Ogata, Y.: Genetic stability of various resistance factors in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 102, 363–368 (1970)
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Communicated by W. Arber
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Lane, D., Chandler, M., Silver, L. et al. The construction and replication properties of hybrid plasmids composed of the r-determinant of R100.1 and the plasmids pCRI or pSC201. Molec. Gen. Genet. 168, 337–340 (1979). https://doi.org/10.1007/BF00271505
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DOI: https://doi.org/10.1007/BF00271505