Summary
Hybrid ColE1 plasmids called ColE1-cosλ-guaA or ColE1-cosλ-gal can be efficiently transduced into various E. coli K-12 cells through packaging into λ phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E. coli K-12 mutants. (1) The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cosλ-guaA. (2) Pre-existing hybrid ColE1 plasmids had no effect on the frequency of λ phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. (3) ColE1-cosλ-guaA and ColE1-cosλ-gal DNAs could temporarily but not stably co-exist in E. coli K-12 recA cells. (4) The presence of ColE1-cosλ-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cosλ-guaA about 7-fold. (5) The same ColE1-cosλ-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA + gen function(s) and suggest that ColE1 plasmid itself provides no recA +-like functions.
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Kibe, A., Shimada, K. & Takagi, Y. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis. Molec. Gen. Genet. 168, 293–298 (1979). https://doi.org/10.1007/BF00271499
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DOI: https://doi.org/10.1007/BF00271499