Summary
Construction of translational fusions betwen the repA gene of plasmid R 1 (required for replication) and the lacZ gene has allowed a quantitative analysis of expression of this gene. It is suggested that the replication of R 1 is controlled by two replication control functions acting as inhibitors of repA expression.
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References
An G, Fricsen JD (1979) Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol 140:400–407
Andres I, Slocombe PM, Cabello F, Timmis JK, Lurz R, Burkhardt HJ, Timmis KN (1979) Plasmid replication functions. II. Cloning analysis of the RepA replication region of antibiotic resistance plasmid R6-5. Mol Gen Genet 168:1–25
Beard JP, Connolly JC (1975) Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells earrying a derepressed F-like R-factor. J Bacteriol 122:59–65
Bertani G (1951) Studies of lysogensis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62:293–300
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
Broda P (1979) Plasmids. Freeman, Oxford and San Francisco
Casadaban MJ, Cohen SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179–207
Casadaban MJ, Chou J, Cohen SN (1980) In vitro gene fusions that join an enzymatically active β-galactosidase segment to aminoterminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol 143:971–980
Clark JD, Maaløe O (1967) DNA replication and the cell cycle in Escherichia coli. J Mol Biol 23:99–112
Cohen SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
Falkow S (1975) Infectious Multiple Drug Resistance. Pion, London
Kollek R, Oertel W, Goebel W (1978) Isolation and characterization of the minimal fragment required for autonomous replication (“Basic Replicon”) of a copy mutant (pKN102) of the antibiotic resistance factor R 1. Mol Gen Genet 162:51–57
Meacock PA, Cohen SN (1979) Genetic analysis of the interrelationship between plasmid replication and incompatibility. Mol Gen Genet 174:135–147
Miki T, Easton AM, Rownd RH (1980) Cloning of replication, incompatibility, and stability functions of R plasmid NR1. J Bacteriol 141:87–99
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Molin S, Stougaard P, Uhlin BE, Gustafsson P, Nordström K (1979) Clustering of genes involved in replication, incompatibility, and stable maintenance of the resistance plasmid R1 drd-19. J Bacteriol 138:70–79
Molin S, Nordström K (1980) Control of plasmid R 1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication. J Bacteriol 141:111–120
Molin S, Stougaard P, Light L, Nordström M, Nordström K (1981) Isolation and characterization of new copy mutants of plasmid R 1 and identification of a polypeptide involved in copy number control. Mol Gen Genet 181:123–130
Nordström K, Ingram LC, Lundbäck A (1972) Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J Bacteriol 110:562–569
Oertel W, Kollek R, Beck E, Goebel W (1979) The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R 1drd-19. Mol Gen Genet 171:277–285
Pritchard RH, Barth PT, Collins J (1969) Control of DNA synthesis in bacteria. Symp Soc Gen Microbiol 19:263–297
Rosen J, Ryder T, Inokuchi H, Ohtsubo H, Ohtsubo E (1980) Genes and sites in replication and incompatibility of an R 100 plasmid derivative based on nucleotide sequence analysis. Mol Gen Genet 179:527–537
Rosen J, Ryder T, Ohtsubo H, Ohtsubo E (1981) Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives. Nature 290:794–797
Stougaard P, Molin S, Nordström K, Hansen FG (1981a) The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19. Mol Gen Genet 181:116–122
Stougaard P, Molin S, Nordström K (1981b) RNA molecules involved in copy number control and incompatibility of plasmid R1. Proc Natl Acad Sci USA (in press)
Taylor DP, Cohen SN (1979) Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy mutant NR1. J Bacteriol 137:92–104
Timmis KN, Andres I, Slocombe PM (1978) Plasmid incompatibility: Cloning analysis of an incFII determinant of R6-5. Nature 273:27–32
Uhlin BE, Nordström K (1975) Plasmid incompatibility and control of replication: Copy mutants of the R-factor R 1 in Escherichia coli K-12. J Bacteriol 124:641–649
Veltkamp E, Nijkamp HJJ (1976) Characterization of a replication mutant of the bacteriocinogenic plasmid Clo DF13. Biochim Biophys Acta 425:356–367
Yoshikawa M (1974) Identification and mapping of the replication genes of an R-factor, R100-1, integrated into the host chromosomes of Escherichia coli K-12. J Bacteriol 118:1123–1131
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Communicated by J. Schell
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Light, J., Molin, S. Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication. Molec. Gen. Genet. 184, 56–61 (1981). https://doi.org/10.1007/BF00271195
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DOI: https://doi.org/10.1007/BF00271195