Summary
An ultrastructural study has been carried out of the changes observed proximal to a nerve constriction in rabbits. The lesion was experimentally produced with a silk ligature. Specimens were examined 24 h after ligation. Significant alterations of the ultra structure were found in sections taken at approximately 1 mm above the constriction. Examination revealed swelling of the nerve fibres due to failure of axoplasmic flow, a marked increase in the number of mitochondria, most of which showed degenerative changes and degenerative changes in the myelin sheath.
Résumé
Les auteurs ont réalisé une étude ultrastructurale des modifications observées dans la partie proximale d'un nerf de lapin soumis à une constriction. La lésion est produite expérimentalement par ligature à la soie. Les échantillons sont observés après 24 heures. Des altérations significatives de l'ultrastructure sont mises en évidence dans des sections faites environ 1 mm en amont de la constriction. Il existe un gonflement des fibres nerveuses, dû au dysfonctionnement du flux axoplasmique, une augmentation marquée du nombre de mitochondries dont la plupart présentent des lésions dégénératives de même que la gaine de myéline.
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References
Babel, J., Bishoff, A., Spoendlin, H.: Ultrastructure of the peripheral nervous system and sense organs. Stuttgart: Bishoff 1970
Catalano, F., Logroscino, C. A.: Studio del flusso assoplasmatico con impiego di radioisotopi e sue modificazioni in rapporto ad alcuni tipi di lesioni traumatiche dei nervi periferici. La Clinica Ortopedica 23, 154–163 (1971–72)
Hebb, C. O., Silver, A.: Gradient of choline acetylase activity. Nature 189, 123–125 (1961)
Kapeller, K., Mayor, D.: An electron microscopic study of the early changes proximal to a constriction in sympathetic nerves. Proc. R. Soc. Lond. [Biol.] 172, 53–63 (1969)
Kidwai, M., Ochs, S.: Components of fast and slow phases of axoplasmic flow. J. Neurochem. 16, 1105–1112 (1969)
Koenig, E., Koelle, G. B.: Mode of regeneration of acetyl-cholinesterase in cholinergic neurons following irreversible inactivation. J. Neurochem. 8, 169–188 (1951)
Lasek, R. J.: Axoplasmic transport in cat dorsal root ganglion cells: As studied with 1 — leucine — h3. Brain Res. 7, 360–377 (1968)
Logroscino, C. A., Catalano, F.: Studio delle modificazioni ultrastrutturaliprecoci, secondarie a compressione sperimentale del nervo tibiale di coniglio. Ital. J. Orthop. Traumatol. 2, 117–133 (1976)
Logroscino, C. A., Catalano, F., Sacchettoni Logroscino, G.: Valutazione critica dell'intervento di neurolisi esterna in corso di assonostenosi sperimentale nel coniglio. Rilievi ulstrutturali Chirurgia Org. Mov. 63, 301–313 (1974)
Lubinska, L.: Axoplasmic streaming in regeneration and in normal nerve fibres. Prog. Brain Res. 13, 1–71 (1964)
Lumsden, C. E.: Quantitative studies on lipolytic enzyme activity in degenerating and regenerating nerve. J. Exp. Physiol. 37, 45–57 (1952)
Miani, N.: Proximo distal movement of phospholipids in the axoplasm of the intact and regenerating neurons. Prog. Brain Res. 13, 115–126 (1964)
Miani, N.: Proximo-distal movement along the axon of protein synthesized in the perikarion of regenerating neurons. Nature 185, 541 (1960)
Ochs, S.: Metabolic dependence of fast axoplasmic transport in nerve. Science 167, 878–879 (1969)
Ochs, S., Johnson, J.: Protein incorporation and axoplasmic flow in motoneurone fibres following intra-cord injection of labelled leucine. J. Neurochem. 14, 317–331 (1967)967)
Sanguinetti, C.: Fisiopatologia delle lesioni nervose. Relaz. 56° Congr. S. I. O. T. Roma 1971
Weiss, P.: Endoneurial edema in constricted nerve. Anat. Rec. 86, 491–522 (1943)
Weiss, P.: Nerve regeneration in the rat following tubular splicing of severed nerves. Arch. Surg. 46, 525–548 (1943)
Weiss, P., Hiscoe, M. B.: Experiments on the mechanism of nerve growth. J. Exp. Zool. 107, 315–395 (1948)
Weiss, P., Pillai, M. B.: Connection and fate of mitochondria in nerve fibres: Axonal flow as vehicle. Proc. Natl. Acad. Sci. USA 54, 48–56 (1965)
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Logroscino, C.A., Sanguinetti, C. & Catalano, F. Electron microscopic study of the early changes proximal to a constriction in myelinated nerve. International Orthopaedics 4, 121–125 (1980). https://doi.org/10.1007/BF00271095
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DOI: https://doi.org/10.1007/BF00271095