Summary
The internal pH of Saccharomyces cerevisiae IGC 3507 III (a respiratory-deficient mutant) was measured by the distribution of [14C]propionic acid, when the yeast was fermenting glucose at pH 3.5, 4.5 and 5.5 in the presence of several concentrations of acetic acid and ethanol. Good correlation was obtained between fermentation rates and internal pH. For all external pH values tested, the internal pH was 7.0–7.2 in the absence of inhibitors. The addition of acetic acid and/or ethanol resulted in a decrease of fermentation rate together with a drop in internal pH. Internal pH did not depend on the concentration of total external acetic acid but only on the concentration of the undissociated form of the acid. Ethanol potentiated the effect of acetic acid both with respect to inhibition of fermentation and internal acidification.
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References
Cássio F, Leão C, Uden N van (1987) Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol 53:509–513
Guijarro JM, Lagunas R (1984) Saccharomyces cerevisiae does not accumulate ethanol against a concentration gradient. J Bacteriol 160:874–878
Lafon-Lafourcade S, Geneix C, Ribéreau-Gayon P (1984) Inhibition of alcoholic fermentation of grape must by fatty acids produced by yeast and their elimination by yeast ghosts. Appl Environ Microbiol 47:1246–1249
Leão C, Uden N van (1984) Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 774:43–48
Loureiro V, Ferreira HG (1983) On the intracellular accumulation of ethanol in yeast. Biotechnol Bioeng 25:2263–2269
Nagodawithana TW, Steinkraus KH (1976) Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in “rapid fermentation”. Appl Environ Microbiol 31:158–162
Novak M, Strehaiano P, Moreno M, Goma G (1981) Alcoholic fermentation: on the inhibitory effect of ethanol. Biotechnol Bioeng 23:201–211
Pinto I, Cardoso H, Leão C, van Uden N (1989) High enthalpy and low enthalpy death in Saccharomyces cerevisiae induced by acetic acid. Biotechnol Bioeng 33:0000–0000
Rottenberg H (1979) The measurement of membrane potential and ΔpH in cells, organelles and vesicles. In: Fleischer S, Packer L (eds) Methods in enzymology, vol 55. Academic Press, London, pp 547–569
Serrano R (1988) Structure and function of proton translocating ATPase in plasma membranes of plants and fungi. Biochim Biophys Acta 947:1–28
Uden N van (1967) Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168
Uden N van (1985) Ethanol toxicity and ethanol tolerance in yeasts. In: Tsao GT (ed) Annual reports on fermentation processes, vol 8. Academic Press, London, pp 11–58
Umbreit WW, Burris RH, Stauffer JF (1964) Manometric techniques, 4th edn. Burgess Publishing Company, Minneapolis
Viegas CA, Sá-Correia I, Novais JM (1985) Synergistic inhibition of the growth of Saccharomyces bayanus by ethanol and octanoic or decanoic acids. Biotechnol Lett 7:611–614
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Pampulha, M.E., Loureiro-Dias, M.C. Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast. Appl Microbiol Biotechnol 31, 547–550 (1989). https://doi.org/10.1007/BF00270792
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DOI: https://doi.org/10.1007/BF00270792