Summary
The DNA sequence has been determined at the sites of three independent occurrences of the transposable element Tn5 in the hisG gene of the histidine operon. All three insertion sites are adjacent to G+C-rich stretches of DNA. In all three cases sequences at the sites of insertion show homology with a region near the ends of Tn5.
References
Barnes WM (1978) DNA sequence from the histidine operon control region: Seven histidine codons in a row. Proc Natl Acad Sci USA 75:4281–4285
Barnes WM (1979) Construction of an M13 histidine-transducing phage: A single-stranded cloning vehicle with one EcoRI site. Gene 5:127–139
Berg DE (1977) Insertion and excision of the transposable kanamycin resistance determinant Tn5. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, New York, pp 205–212
Berg DE, Weiss A, Crossland L (1980) Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol 142:439–446
Bossi L, Roth JR (1980) The influence of codon context on genetic code translation. Nature 286:123–127
Bossi L, Roth JR (1981) Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ. Cell, in press
Calos MP, Miller JH (1980) Transposable elements. Cell 20:579–595
Calos MP, Johnsrud L, Miller JH (1978) DNA sequence at the integration sites of the insertion element ISI. Cell 13:411–418
Chumley FG, Menzel R, Roth JR (1979) Hfr formation directed by Tn10. Genetics 91:639–655
Chumley FG, Roth JR (1980) Rearrangement of the bacterial chromosome using Tn10 as a region of homology. Genetics 94:1–14
Galas DJ, Calos MP, Miller JH (1980) Sequence analysis of Tn9 insertions in the lacZ gene. J Mol Biol 144:19–41
Grindley NDF (1978) ISI insertion generates duplication of a nine base pair sequence at its target site. Cell 13:419–426
Hoppe I, Johnston HM, Biek D, Roth JR (1978) A refined map of the hisG gene of Salmonella typhimurium. Genetics 92:17–26
Johnston HM, Roth JR (1981) DNA sequence changes of mutations altering attenuation control of the histidine operon of Salmonella typhimurium. J Mol Biol 145:735–756
Kleckner N (1979) DNA sequences analysis of Tn10 insertions: Origin and role of 9 bp flanking repetitions during Tn10 translocation. Cell 16:711–720
Kleckner N, Roth JR, Botstein D (1977) Genetic engineering in vivo using translocatable drug-resistance elements. J Mol Biol 116:125–159
Kuhn S, Fritz H-J, Starlinger P (1979) Close vicinity of ISI integration sites in the leader sequence of the gal operon of E. coli. Mol Gen Genet 167:235–241
Miller JH, Calos MP, Galas DJ, Hofer M, Büchel D, Müller-Hill B (1980) Genetic analysis of transpositions in the lac region of Escherichia coli. J Mol Biol 144:1–18
Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schaller H (1978) The intergenic region and the origin for filamentous phage DNA replication. Cold Spring Harbor Symp Quant Biol 43:401–408
Schmid M, Roth JR (1980) Circularization of transduced fragments: A mechanism for adding segments to the bacterial chromosome. Genetics 94:15–29
Starlinger P, Saedler H (1976) IS elements in microorganisms. Curr Top Microbiol Immunol 75:111–152
Tu C-PD, Cohen SN (1980) Translocation specificity of the Tn3 element: Characterization of sites of multiple insertions. Cell 19:151–160
Vogel HJ, Bonner DM (1956) Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218:97–106
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Bossi, L., Ciampi, M.S. DNA sequences at the sites of three insertions of the transposable element Tn5 in the histidine operon of Salmonella . Molec. Gen. Genet. 183, 406–408 (1981). https://doi.org/10.1007/BF00270649
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270649