Summary
The effects of different tnm mutations on the transposition of Tn3, Tn5, Tn10, Tn601, and bacteriophage Mu were studied. Five tnm mutations were placed into three phenotypic classes. The representatives of the first class, tnm-1 and tnm-2, caused a complete block of transposition of all Tn-elements studied; the representatives of the second class, tnm-3 and tnm-4, specifically affected Tn9 transposition while the tnm-5 mutation attributed to the third class caused formation of cointegrates between the donor genome of phage λ carrying Tn-elements Tn3 or Tn5 and the recipient genome (bacterial chromosome).
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References
Arthur A, Sherratt D (1979) Dissection of the transposition process: a transposon-encoded site specific recombination system. Mol Gen Genet 175:267–274
Berg DE (1977) Detection of transposable antibiotic resistance determinants with phage lambda. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 553–558
Gill R, Heffron F, Dougan G, Falkow S (1978) Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol 136:742–756
Hyina TS, Nechaeva EV, Belikov NG, Smirnov GB (1978) The specificity of the insertion of the transposon controlling chloramphenicol resistance (Tn9) into Escherichia coli K12 chromosome. Genetics (USSR). 14:2071–2081
Hyina TS, Nechaeva EV, Pasynkova LN, Smirnova NI, Smirnov GB (1980a) Tn9 integration sites and their influence on the transposon properties. Genetics (USSR) 16:46–54
Hyina TS, Nechaeva EV, Smirnov GB (1980b) The method of isolation and initial characterization of bacterial mutants with altered ability to transpose Tn9. Genetics (USSR) 16:957–966
Ilyina TS, Nechaeva EV, Romanova YuM, Smirnov GB (1981) Isolation and mapping of Escherichia coli K12 mutants defective in Tn9 transposition. Mol Gen Genet 181:384–389
Kleckner N, Barker DF, Ross DG, Botstein D (1978) Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics 90:427–461
Meyer R, Boch G, Shapiro J (1979) Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element. Mol Gen Genet 171:7–13
Shapiro J (1979) Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci USA 76:1933–1937
Smirnov GB, Ilyina TS, Romanova Yu M, Markov AP, Nechaeva EV (1981) Mutants of Escherichia coli affected in the processes of transposition and genomic rearrangements. Cold Spring Harbor Symp Quant Biol 45 (in press)
Weinstock GM, Botstein D (1979) Regional specificity of illegitimate recombination associated with the translocatable ampicillin-resistance element Tn1. Cold Spring Harbor Symp Quant Biol 43:1209–1215
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Communicated by H. Saedler
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Ilyina, T.S., Romanova, Y.M. & Smirnov, G.B. The effect of tnm mutations of Escherichia coli K12 on transposition of various movable genetic elements. Molec. Gen. Genet. 183, 376–379 (1981). https://doi.org/10.1007/BF00270643
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DOI: https://doi.org/10.1007/BF00270643