Summary
Primary cell cultures derived from embryos of a control stock of Drosophila melanogaster respond to ultraviolet light within the first hour after exposure with a decline in thymidine incorporation and a decline in the ability to form newly synthesized (nascent) DNA in long segments. Cells derived from two nonallelic excision-defective mutants (mei-9 and mus201) exhibit the same quantitative decline in both phenomena as do control cells. In contrast, cells from five nonallelic postreplication repair-defective mutants (mei-41, mus101, mus205, mus302 and mus310) respond to ultraviolet light by synthesizing nascent DNA in abnormally short segments. Two of these five mutants (mus302 and mus310) also exhibit unusually low thymidine incorporation levels after irradiation, whereas the other three mutants display the normal depression of incorporation.
These results indicate that excision repair does not influence the amount or the length of nascent DNA synthesized in Drosophila cells within the first hour after exposure to ultraviolet light. Of the five mutations that diminish postreplication repair, only two reduce the ability of irradiated cells to synthesize normal amounts of DNA.
Similar content being viewed by others
Abbreviations
- UV:
-
ultraviolet light — principal wavelength 254 nm
References
Blumenthal AB, Kriegstein HJ, Hogness DS (1973) The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harbor Symp Quant Biol 38:205–223
Boyd JB, Setlow RB (1976) Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. Genetics 84:507–526
Boyd JB, Golino MD, Setlow RB (1976) The mei-9 a mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. Genetics 84:527–544
Boyd JB, Harris PV, Osgood CJ, Smith KE (1980) Biochemical characterization of repair-deficient mutants of Drosophila. In: Generoso WM, Shelby MD, de Serres FJ (eds) DNA repair and mutagenesis in eucaryotes, Plenum, New York 209–221
Brown TC, Boyd JB (1981) Abnormal recovery of DNA replication in ultraviolet-irradiated cell cultures of Drosophila melanogaster which are defective in DNA repair. Mol Gen Genet 183:363–368
Caillet-Fauquet P, Defais M, Radman M (1977) Molecular mechanisms of induced mutagenesis. Replication in vivo of bacteriophage ΣX174 single-stranded, ultraviolet light-irradiated DNA in intact and irradiated host cells. J Mol Biol 117:95–112
Chan LN, Gehring W (1971) Determination of blastoderm cells in Drosophila melanogaster. Proc Natl Acad Sci USA 68:2217–2221
Cleaver JE (1967) The relationship between the rate of DNA synthesis and its inhibition by ultraviolet light in mammalina cells. Radiat Res 30:795–810
Cleaver JE (1978) DNA repair and its coupling to DNA replication in eucaryotic cells. Biochim Biophys Acta 516:489–516
Cordeiro-Stone M, Shumacher RI, Meneghini R (1979) Structure of the replication fork in ultraviolet light-irradiated human cells. Biophys J 27:287–300
Doniger J (1978) DNA replication in ultraviolet light irradiated Chinese hamster cells: The nature of replicon inhibition and post-replication repair. J Mol Biol 120:433–446
Edenberg HE (1976) Inhibition of DNA replication by ultraviolet light. Biophys J 16:849–860
Hand R (1978) Eucaryotic DNA: Organization of the genome for replication. Cell 15:317–325
Harris PV, Boyd JB (1980) Excision repair in Drosophila. Analysis of strand breaks appearing in DNA of mei-9 mutants following mutagen treatment. Biochem Biophys Acta 610:116–129
Higgins NP, Kato K, Strauss B (1976) A model for replication repair in mammalian cells. J Mol Biol 101:417–425
Lavin MF (1978) Postreplication repair in mammalian cells after ultraviolet irradiation. A model. Biophys J 23:247–255
Lehmann AR (1976) Postreplication repair of DNA in mammalian cells: a discussion of the mechanisms and biological importance. In: Kiefer J (ed) Radiation and cellular control processes. Springer, New York, pp 147–158
Lehmann AR (1979) The relationship between pyrimidine dimers and replicating DNA in UV-irradiated human fibroblasts. Nucl Acids Res 7:1901–1912
Lehmann AR, Kirk-Bell S, Arlett CF, Paterson MC, Lohman PHM, de Weerd-Kastelein EA, Bootsma D (1975) Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation. Proc Natl Acad Sci USA 72:219–223
Meneghini R, Hanawalt P (1976) T4-endonuclease V-sensitive sites in DNA from ultraviolet-irradiated human cells. Biochem Biophys Acta 425:428–437
Meyn RE, Hewitt RR, Thomson LF, Humphrey RM (1976) Effects of ultraviolet irradiation on the rate and sequence of DNA replication in synchronized Chinese hamster cells. Biophys J 16:517–525
Nguyen TD, Boyd JB (1977) The meiotic-9 (mei-9) mutants of Drosophila melanogaster are deficient in repair replication of DNA. Mol Gen Genet 158:141–147
Park SD, Cleaver JE (1979a) Recovery of DNA synthesis after ultraviolet irradiation of xeroderma pigmentosum cells depends on excision repair and is blocked by caffeine. Nucl Acids Res 6:1151–1159
Park SD, Cleaver JE (1979b) Postreplication repair: questions of its definition and possible alteration in xeroderma pigmentosum cell strains. Proc Natl Acad Sci USA 76:3927–3931
Rudé JM, Friedberg EC (1977) Semi-conservative deoxyribonucleic acid synthesis in unirradiated and ultraviolet-irradiated xeroderma pigmentosum and normal human skin fibroblasts. Mutat Res 42:433–442
Shalek RJ, Sinclair WK, Calkins JC (1962) The relative biological effectiveness of 22-Mevp X-rays, cobalt-60 gamma rays, and 200-Krep X-rays. II. The use of the ferrous sulfate dosimeter for X-ray and gamma ray beams. Radiat Res 16:344–351
Waters R (1979) Repair of DNA in replicated and unreplicated portions of the human genome. J Mol Biol 127:117–127
Author information
Authors and Affiliations
Additional information
Communicated by B.A. Bridges
Rights and permissions
About this article
Cite this article
Brown, T.C., Boyd, J.B. Postreplication repair-defective mutants of Drosophila melanogaster fall into two classes. Molec. Gen. Genet. 183, 356–362 (1981). https://doi.org/10.1007/BF00270640
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270640