Summary
In some yeast cytoplasmic mutants, ethidium bromide resistance is expressed at the mitochondrial level. In homosexual crosses it leads to an enhanced recombination between two linked mitochondrial genes controlling chloramphenicol and erythromycin resistance or sensitivity, but recombination is still reciprocal. When ethidium bromide resistant cells are irradiated with U.V., the recovery of intact markers out of irradiated mitochondrial genomes is increased by comparison with that of ethidium bromide sensitive cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avner, P. R., Coen, D., Dujon, B., Slonimski, P. P.: Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant in Saccharomyces cerevisiae. Molec. gen. Genet. 125, 9–53 (1973)
Avner, P. R., Griffiths, D. E.: Oligomycin resistant mutant in yeast. FEBS Letters 10, 202–207 (1970)
Bech-Hansen, N. T., Rank, G. H.: Ethidium bromide resistance and petite induction in Saccharomyces cerevisiae. Canad. J. Genet. Cytol. 14, 681–689 (1972)
Bolotin, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., Slonimski, P. P.: La recombinaison des mitochondries chez Saccharomyces cerevisiae Bull. Inst. Pasteur (Paris) 69, 215–239 (1971)
Brunner, A., Mas, J., Celis, E., Mattoon, J. R.: Cytoplasmic and nuclear inheritance of resistance to alkylguanidine and ethidium bromide in a petite-negative yeast. Biochem. Biophys. Res. Commun 53, 638–645 (1973)
Bunn, C. L., Mitchell, C. H., Lunnane, A. W.: Biogenesis of mitochondria XVIII. A new class of cytoplasmically determined antibiotic resistant mutants in Saccharomyces serevisiae. Proc. nat. Acad. Sci. (Paris) 67, 1233–1240 (1970)
Crandall, M.: A respiratory-deficient mutant in the obligately aerobic yeast. Hansennula Wingei. J. gen. Microbiol. 75, 337–381 (1973)
Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., Slonimski, P. P., Bolotin-Fukuhara, M., Coen, D.: Mitochondrial genetics VI the petite mutation in Saccharomyces cerevisiae: Interrelation between the loss of the ϱ+ factor and the loss of the drug resistance mitochondrial genetic markers. Molec. gen. Genet. (in press)
Goldfarb, D. M., Goldberg, G. I., Chernin, L. S., Gilkova, L. A., Avdienko, I. D., Kuznetsova, B. N., Kushner, I. C.: A protein producted by male strains of E. coli K-12 which increases the yield of recombinants in conjugation: its nature and mode of action. Molec. gen. Genet. 120, 211–226 (1973)
Goldring, E. S., Grossman, L. F., Marmur, J.: Petite mutation in yeast II. Isolation of mutants containing mitochondrial desoxyribonucleic acid of reduced size. J. Bact. 107, 377 (1971)
Gouhier, M., Mounolou, J. C.: Yeast mutants resistant to ethidium bromide. Molec. gen. Genet. 122, 149–164 (1973)
Howell, N., Trembath, M. K., Linnane, A. W., Lukins, H. B.: Biogenesis of mitochondria 30. An analysis of polarity of mitochondrial gene recombination and transmission. Molec. gen. Genet. 122 37–51 (1973)
Kleese, R. A., Grotbeck, R. C., Snyder, S. R.: Recombination among three mitochondrial genes in yeast (Saccharomyces cerevisiae). J. Bact. 112, 1023–1025 (1972)
Klietmann, W., Sato, N., Nass, M. M. K.: Establishment and characterization of ethidium bromide resistance in Simian virus 40 transformed hamster cells. J. Cell Biol. 58, 11–26 (1973)
Kutzleb, R., Schweyen, R. J., Kaudewitz, F.: Extrachromosomal inheritance of paromomiyen resistance in Saccharomyces cerevisiae. Genetic and biochemical characterization of mutants. Molec. gen. Genet. 125, 91–98 (1973)
Mahler, H. R., Perlman, P. S.: Mitochondrial membrane and mutagenesis by ethidium bromide. J. supramol. Struct. 1, 105–124 (1972)
Michaelis, G., Petrochilo, E., Slonimski, P. P.: Mitochondrial genetics. III. Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of Saccharomyces cerevisiae: physical and genetic characterization. Molec. gen. Genet. 125, 51–65 (1973)
Paoletti, C., Couder, H., Guerineau, M.: A yeast mitochondrial desoxyribonuclease stimulated by ethidium bromide. Biochem. biophys. Res. Commun. 48, 950–958 (1972)
Perlman, P. S., Mahler, H. R.: Molecular consequence of ethidium bromide mutagenesis. Nature (Lond.) 231, 12–16 (1971)
Slonimsky, P. P., Perrodin, G., Croft, J.: Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal “petite”. Biochem. biophys. Res. Commun. 30, 232 (1968)
Thomas, D. Y., Wilkie, D.: Recombination of mitochondrial drug resistance factors in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968)
Trembath, M. K., Bunn, C. L., Kukins, N. B., Linnane, A. W.: Biogenesis of mitochondria 27. Genetic and biochemical characterization of cytoplasmic and nuclear mutations to spiramycin resistance in Saccharomyces cerevisiae. Molec. gen. Genet. 121, 35–48 (1973)
Wolf, K., Dujon, B., Slonimski, P. P.: Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation confering paromomycin resistance in Saccharomyces cerevisiae. Molec. gen. Genet. 121, 53–90 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Gouhier-Monnerot, M. Ethidium bromide resistance and enhancement of mitochondrial recombination. Molec. Gen. Genet. 130, 65–79 (1974). https://doi.org/10.1007/BF00270519
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270519