Summary
Exonuclease V, the ATP-dependent exonuclease which is a product of the recB and recC genes, is not responsible for the excessive UV-induced DNA degradation in polA mutants since cells mutant at both polA and recB retain the ability to degrade their DNA after UV irradiation. It is proposed that the excessive degradation in polA mutants could be due to uncontrolled 5′ to 3′ exonuclease activity of the amber fragment of DNA polymerase I. An ATP-independent exonuclease which appears in recB mutants suppressed by sbcA mutations does not restore the ability of recB mutants to degrade their irradiated DNA. UV induced DNA degradation in E. coli cells made permeable to small molecules requires an ATP dependent, NEM sensitive function which may be an endonuclease that acts early in the repair process.
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Communicated by B. A. Bridges
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Strike, P., Emmerson, P.T. Degradation of DNA following ultraviolet irradiation of Escherichia coli K12 Mutants lacking DNA polymerase I and exonuclease V. Molec. Gen. Genet. 130, 39–45 (1974). https://doi.org/10.1007/BF00270517
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DOI: https://doi.org/10.1007/BF00270517