Summary
Cold sensitive (cs) mutants of E. coli unable to grow at 26° C were isolated. Three of about 200 mutants tested have RNA polymerase which is about 5 times less active at low temperatures than the wild type RNA polymerase. One of such mutants cs 1 was studied in more detail. The mutation affects core enzyme and is closely linked to rif-r mutations. By transduction analysis the following order of markers was established: argH — rif-r — cs 1.
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Nikiforov, V.G., Kalyaeva, E.S. & Velkov, V.V. Cold sensitive mutant of Escherichia coli with altered RNA polymerase. Molec. Gen. Genet. 130, 1–7 (1974). https://doi.org/10.1007/BF00270513
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DOI: https://doi.org/10.1007/BF00270513