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Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids

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Summary

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.

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Authors and Affiliations

  1. Institute for Genetics, University of Cologne, Weyertal 121, D-5000, Cologne 41, Federal Republic of Germany

    Ulrich Rüther

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  1. Ulrich Rüther
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Communicated by E. Bautz

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Rüther, U. Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids. Molec. Gen. Genet. 178, 475–477 (1980). https://doi.org/10.1007/BF00270503

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  • DOI: https://doi.org/10.1007/BF00270503

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