Skip to main content
SpringerLink
Log in

On the role of the dnaB protein of Escherichia coli in the replication of λ bacteriophage DNA

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The interaction between the dnaB protein of E. coli and the gene P product of λ bacteriophage was investigated by measuring the cleavage of closed circular phage DNA after infection of two temperature sensitive dnaB mutants, JG28 and To534 groP - B. Cleavage of superhelical DNA from a λπB mutant phage was observed after infection of either strain whereas superhelical DNA from a wild type phage was only cleaved after infection of JG28. When DNA synthesis in infected cells was blocked by incubation at the nonpermissive temperature, no inhibition of superhelical phage DNA cleavage was observed. It is concluded that in conditions where the dnaB protein has lost the capacity to function in nucleotide polymerization, it is capable of interacting with the λ replication gene products to introduce a break in the phage DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • D'Ari, R., Jaffe-Brachet, A., Touati-Schwartz, D., Yarmolinsky, B.: A dnaB analog specified by bacteriophage P1. J. Mol. Biol. 94, 341–366 (1975)

    Google Scholar 

  • Freifelder, D., Kirschner, I.: A phage λ endonuclease controlled by genes O and P. Virology 44, 223–225 (1971)

    Google Scholar 

  • Georgopolous, C.P., Herskowitz, I.: E. coli mutants blocked in λ DNA synthesis. In: Bacteriophage lambda (A. Hershey, ed.), pp. 553–564. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Kogoma, T.: Two types of temperature sensitivity in DNA replication of an Escherichia coli dnaB mutant. J. Mol. Biol. 103, 191–197 (1976)

    Google Scholar 

  • Lanka, E., Mikolajczyk, M., Schlich, M., Schuster, H.: Association of the prophage P1 ban protein with the dnaB protein of Escherichia coli. J. Biol. Chem. 253, 4746–4753 (1978)

    Google Scholar 

  • Lanka, E., Schuster, H.: Replication of bacteriophages in Escherichia coli mutants thermosensitive in DNA synthesis. Mol. Gen. Genet. 106, 274–285 (1970)

    Google Scholar 

  • Lark, K.G., Wechsler, J.A.: DNA replication in dnaB mutants of Escherichia coli: Gene product interaction and synthesis of 4S pieces. J. Mol. Biol. 92, 145–163 (1975)

    Google Scholar 

  • McMacken, R., Kessler, S., Boyce, R.: Strand breakage of λ DNA supercoils in infected Lysogens. 1. Genetic and biochemical evidence for two types of nicking processes. Virology 66, 356–371 (1975)

    Google Scholar 

  • Ogawa, T.: Analysis of the dnaB function of Escherichia coli K12 and the dnaB-like function of P1 prophage. J. Mol. Biol. 94, 327–340 (1975)

    Google Scholar 

  • Pricer, W.B., Jr., Weissbach, A.: The effect of lysogenic induction with mitomycin C on the deoxyribonucleic acid polymerase of Escherichia coli K 12 (λ). J. Biol. Chem. 239, 2607 (1964)

    Google Scholar 

  • Saito, H., Uchida, H.: Initiation of the DNA replication of bacteriophage λ in Escherichia coli K12. J. Mol. Biol. 113, 1–25 (1977)

    Google Scholar 

  • Saito, H., Uchida, H.: Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. Mol. Gen. Genet. 164, 1–8 (1978)

    Google Scholar 

  • Salzmann, L.A., Weissbach, A.: Studies on the formation of circular λ DNA. Virology 31, 170–177 (1966a)

    Google Scholar 

  • Salzman, L.A., Weissbach, A.: Formation of intermediates in the replication of phage λ DNA. J. Mol. Biol. 28, 53–70 (1966b)

    Google Scholar 

  • Shuster, R.C.: The effect of chloramphenicol and rifampicin on the “nicking” and “membrane attachment” of bacteriophage λ DNA. Virology 62, 472–482 (1974)

    Google Scholar 

  • Shuster, R.C., Weissbach, A.: Genetic mapping of an endonuclease synthesized by bacteriophage lambda. Nature 223, 852–953 (1969)

    Google Scholar 

  • Skalka, A.M.: DNA replication-bacteriophage lambda. In: Curr. topics microb. and immunol., vol. 78, pp. 201–238. Berlin-Heidelberg-New York: Springer 1977

    Google Scholar 

  • Thirion, J.P.: Étude cinétique du controlé génétique des différents formes d'ADN de λ apres infection. Ann. Inst. Pasteur 120, 453–465 (1971)

    Google Scholar 

  • Tomizawa, J.: Functional cooperation of genes O and P. In: Bacteriophage lambda (A. Hershey, ed.) pp. 549–552. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Ueda, K., McMacken, R., Kornberg, A.: dnaB protein of Escherichia coli: purification and role in the replication of ϕX174 DNA. J. Biol. Chem. 253, 261–269 (1978)

    Google Scholar 

  • Yochem, J., Uchida, H., Sunshine, M., Saito, H., Georgopolous, C.P., Feiss, M.: Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Mol. Gen. Genet. 164, 9–14 (1978)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Emory University School of Medicine, 30322, Atlanta, Georgia, USA

    Robert C. Shuster

Authors
  1. Robert C. Shuster
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by L. Lerman

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shuster, R.C. On the role of the dnaB protein of Escherichia coli in the replication of λ bacteriophage DNA. Molec. Gen. Genet. 178, 429–435 (1980). https://doi.org/10.1007/BF00270495

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270495

Keywords

Search

Navigation