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Neighbour and temperature effects on base-analogue-induced mutation in phage T4

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Summary

The effect of neighbouring base pairs and of temperature on mutation frequencies were measured at nonsense sites in the T4rII region. 2AP-induced AT→GC transition frequencies are insensitive to nearest-neighbour effects, while 5BU-induced ones are promoted by GC neighbours on the 5′ side. The effect of temperature on 2AP-and 5BU-induced mutation frequencies shows no simple dependence on nearest neighbours. These results are incompatible with a unitary mechanism as explanation for the effects of nearest neighbours and temperature on baseanalogue-induced mutagenesis.

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Authors and Affiliations

  1. Department of Genetics, The Hebrew University of Jerusalem, Israel

    Amiram Ronen & Calomira Halevy

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  1. Amiram Ronen
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  2. Calomira Halevy
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Communicated by B.A. Bridges

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Ronen, A., Halevy, C. Neighbour and temperature effects on base-analogue-induced mutation in phage T4. Molec. Gen. Genet. 178, 397–401 (1980). https://doi.org/10.1007/BF00270490

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  • DOI: https://doi.org/10.1007/BF00270490

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