Skip to main content
Log in

Pseudovirulent mutants of λb221poriCasnA resulting from mutations in or near oriC, the E. coli origin of DNA replication

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

Mutants of the specialized transducing phage λb221poriCansA have been isolated which form plaques on lambda lysogens. Genetic and physical evidence is presented to show that the mutations responsible for the pseudovirulent phenotype map in or near oriC, the origin of chromosomal replication in Escherichia coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Backman, K.: Ph.D. thesis. Harvard University, Cambridge (1977)

  • Backman, K., Ptashne, M., Gilbert, W.: Construction of plasmids carrying the cI gene of bacteriophage λ. Proc. Natl. Acad. Sci. U.S.A. 73, 4174–4178 (1976)

    Google Scholar 

  • Backman, K., Ptashne, M.: Maximizing gene expression on a plasmid using recombination in vitro. Cell 13, 65–71 (1978)

    Google Scholar 

  • Bailone, A., Devoret, R.: Isolation of ultravirulent mutants of phage λ. Virology 84, 547–550 (1978)

    Google Scholar 

  • Bolivar, F., Rodriquez, R.L., Betlach, M.C., Boyer, H.W.: Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 2, 75–93 (1977)

    Google Scholar 

  • Georgopoulos, C.P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. In: Bacteriophage lambda (A.D. Hershey, ed.), pp. 553–574. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Hanna, M.H., Carl, P.L.: Reinitiation of deoxyribonucleic acid synthesis by deoxyribonucleic acid initiation mutants of Escherichia coli: role of ribonucleic acid synthesis, protein synthesis, and cell division. J. Bacteriol. 121, 219–226 (1975)

    Google Scholar 

  • Hirota, Y., Mordoh, J., Jacob, F.: On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J. Mol. Biol. 53, 369–387 (1970)

    Google Scholar 

  • Huskey, R.J.: Ph.D.thesis, California Institute of Technology (1967)

  • Jacob, F., Wollman, E.: Etude genetique dun bacteriophage tempere d'Escherichia coli. I. Le systeme genetique du bacteriophage. Ann Inst. Pasteur Lille 87, 653–673 (1954)

    Google Scholar 

  • Lark, K.G., Repko, T., Hoffman, E.J.: The effect of amino acid deprivation on subsequent DNA replication. Biochim. Biophys. Acta 76, 9–24 (1963)

    Google Scholar 

  • Masters, M., Andresdottir, V., Wolfe-Watz, H.: Plasmids carrying oriC can integrate at or near the chromosome origin of E. coli in the absence of a functional recA product. Cold Spring Harbor Symp. Quant. Biol. 43, 1069–1072 (1978)

    Google Scholar 

  • Meijer, M., Beck, E., Hansen, F.G., Bergmans, H.E.N., Messer, W., Meyenburg, K. von, Schaller, H.: Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc. Natl. Acad. Sci. U.S.A. 76, 580–584 (1979)

    Google Scholar 

  • Messer, W., Bergmans H.E.N., Meijer, M., Womack, J.E., Hansen, F.G., Meyenburg, K. von: Mini-chromosomes: Plamids which carry the E. coli replication origin. Mol. Gen. Genet. 162, 269–275 (1978)

    Google Scholar 

  • Miller, J.: Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1972

    Google Scholar 

  • Ordal, G.: Supervirulent mutants and the structure of operator and promoter. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 565–570. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Pereira da Silva, L., Jacob, F.: Etude genetique d'une mutation modifient la sensibilite a l'immunite chez le bacteriophage lambda. Ann. Inst. Pasteur Lille 115, 145–158 (1968)

    Google Scholar 

  • Pritchard, R.H.: Control of DNA replication in bacteria. In: DNA synthesis present and future (Molineaux, I., Kohiyama, M., ed.) pp. 1–26. New York: Plenum 1977

    Google Scholar 

  • Ross, D.G., Swan, I., Kleckner, N.: Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repititions. Cell 16, 721–731 (1979)

    Google Scholar 

  • Soll, L.: Isolation and characterization of λb221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome. Mol. Gen. Genet. 178, 381–389 (1980)

    Google Scholar 

  • Sussman, R., Jacob, F.: Sur un systeme de repression thermosensible chez le bacteriophage λ d'Escherichia coli. Compt. Rend. Acad. Sci. 254, 1517–1519 (1962)

    Google Scholar 

  • Yarus, M.: Isolation and properties of a plasmid which expresses the E. coli Su+7 amber suppressor tRNA gene. Mol. Gen. Genet. 170, 291–298 (1979)

    Google Scholar 

  • Yarus, M., Eisenberg, S.P., Soll, L.: The structure of the DNA containing the E. coli tRNATry gene. Mol. Gen. Genet. 170, 299–308 (1979)

    Google Scholar 

  • Zyskind, J.W., Deen, L.T., Smith, D.W.: Temporal sequence of events during the initiation process in Escherichia coli DNA replication: Roles of the dnaA and dnaC gene products and RNA polymerase. J. Bacteriol. 129, 1466–1475 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H.W. Boyer

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soll, L. Pseudovirulent mutants of λb221poriCasnA resulting from mutations in or near oriC, the E. coli origin of DNA replication. Molec. Gen. Genet. 178, 391–396 (1980). https://doi.org/10.1007/BF00270489

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270489

Keywords

Navigation