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Isolation and characterization of λb221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome

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Summary

A specialized transducing phage, λb221poriCasnA has been isolated carrying oriC the origin of chromosomal replication of Escherichia coli. All phage genes required for lytic growth are retained, thus the phage is capable of lytic growth. The presence of the oriC locus confers upon infecting phage DNA the ability to replicate as a plasmid using only host DNA replication functions. The presence of both oriC and asnA markers has allowed the development of a plaque assay for origin function which can be used to identify mutants at these loci. Comparison of restriction endonuclease cleavage sites present on λb221poriCasnA DNA to those on tis parent, λb221 rex::Tn10 suggests the steps involved in the formation of the transducing phage.

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  1. Department of Molecular, Cellular and Developmental Biology, University of Colorado, 80309, Boulder, Colorado, USA

    Larry Soll

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Communicated by A.W. Boyer

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Soll, L. Isolation and characterization of λb221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome. Molec. Gen. Genet. 178, 381–389 (1980). https://doi.org/10.1007/BF00270488

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  • DOI: https://doi.org/10.1007/BF00270488

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