Summary
The mitochondrial ATPase from oligomycin-resistant mutants which map on different regions of an extrachromosomal DNA (01 and 011 class mutants) showed an increased resistance to oligomycin and venturicidin when assayed in vitro as compared to the sensitive strains.
The resistance to oligomycin of the isolated mitochondrial ATPase from 01 class mutants was higher than that of the 011 class mutants.
Cross resistance of the oligomycin-resistant mutants to the antibiotics peliomycin and ossamycin, which also inhibit phosphoryl transfer reactions in mitochondria (Walter et al., 1967), was observed, 01 mutants being more resistant to ossamycin than 011 class mutants. At the concentrations of peliomycin studied, no difference in sensitivity among both groups of oligomycin-resistant mutants could be detected.
Mitochondrial respiration and isolated mitochondrial ATPase activity are sensitive to venturicidin, suggesting that the previously observed (Brunner et al., 1977) in vivo venturicidin resistance of K. lactis is probably due to an impairment of the influx of the drug at the level of the plasma membrane.
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Avner, P.R., Coen, D., Dujon, B., Slonimski, P.P.: Mitochondrial genetics IV. Allelism and mapping studies of oligomycin-resistant mutants in S. cerevisiae. Mol. Gen. Genet. 125, 9–52 (1973)
Brunner, A., Tuena de Cobos, A., Griffiths, D.E.: The isolation and genetic characterization of extrachromosomal cloramphenicol and oligomycin-resistant mutants from the petite-negative yeast Kluyveromyces lactis. Mol. Gen. Genet. 152, 183–191 (1977)
Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P.P.: Mitochondrial genetics I. Methodology and phenomenology in control of organelle development. Symp. Soc. Exp. Biol. (Miller, P.L., ed.), Vol. 24, pp. 449–496. Cambridge: Cambridge University Press 1970
Gillham, N.: Organelle heredity. New York: Raven Press 1978
Heritage, J., Whittaker, P.A.: Isolation of metabolically active petite mutants of Kluyveromyces lactis a petite-Negative Yeast. Mol. Gen. Genet. 156, 93–98 (1977)
Houghton, R.L., Lancashire, W.E., Griffiths, D.E.: A biochemical genetic investigation of oligomycin and related inhibitors. Biochem. Soc. Trans. 2, 210–213 (1974)
Lancashire, W.E., Griffiths, D.E.: Studies on energy linked reactions: Genetic analysis of venturicidin-resistant mutants. Eur. J. Biochem. 51, 403–413 (1975)
Lardy, H., Reed, P., Chiu-Lin, Ch.: Antibiotic Inhibitors of mitochondrial ATP synthesis. Fed. Proc. 34, 1707–1710 (1975)
Linnane, A.W., Sanders, G.W., Gingold, E.B., Lukins, H.B.: The biogenesis of mitochondria V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cervisiae. Proc. Natl. Acad. Sci. U.S.A. 59, 903–910 (1968)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Luha, A.A., Whittaker, P.A., Hammond, R.C.: Biosynthesis of yeast mitochondria. Some effects of ethidium bromide on Kluyveromyces (Saccharomyces) fragilis. Mol. Gen. Genet. 129, 311–323 (1974)
Mattoon, J.R., Balcavage, W.X.: Yeast mitochondria and submitochondrial particles. In: Methods of enzymology (Estabrook, R.W. and Pullman, M.E., eds.), Vol. 10, pp. 135–142. New York: Academic Press 1967
Mitchell, C.H., Bunn, C.L., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria 23. The biochemical and genetic characteristics of two different oligomycin-resistant mutants of Saccharomyces cerevisiae under the influence of cytoplasmic genetic modification. Bioenergetics 4, 161–177 (1973)
Sager, R.: Cytoplasmic genes and organelles. New York: Academic Press 1972
Schatz, G., Mason, T.L.: The biosynthesis of mitochondrial proteins. Ann. Rev. Biochem. 43, 51–87 (1974)
Shannon, Ch., Enns, R., Wheelis, L., Burchiel, K., Criddle, R.S.: Alterations in mitochondrial adenosine triphosphatase activity resulting from mutation of mitochondrial desoxyribonucleic acid. J. Biol. Chem. 248, 3004–3011 (1973)
Somlo, M., Avner, P.R., Cosson, J., Dujon, B., Krupa, M.: Oligomycin sensitivity of ATPase studied as a function of mitochondrial biogenesis using mitochondrrially determined oligomycinresistant mutants of Saccharomyces cerevisiae. Eur. J. Biochem. 42, 439–445 (1974)
Stuart, K.D.: Cytoplasmic inheritance of oligomycin and rutamycin resistance in yeast. Biochem. Biophys. Res. Commun. 39, 1045–1051 (1970)
Sumner, B.J.: A method for the colorimetric determination of phosphorus. Science 100, 413–414 (1944)
Tzagoloff, A.: Assembly of the mitochondrial membrane system III. Function and synthesis of the oligomycin-sensitivity-conferring protein of yeast mitochondria. J. Biol. Chem. 245, 1545–1551 (1970)
Walter, P., Lardy, H.A., Johnson, D.: Antibiotics as tools for metabolic studies X. Inhibition of phosphoryl transfer reactions in mitochondria by peliomycin, ossamycin and venturicidin. J. Biol. Chem. 242, 5014–5018 (1967)
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Brunner L., A., de Cobos, A.T. Extrachromosomal oligomycin-resistant mutants of the petite-negative yeast Kluyveromyces lactis. Properties of mitochondrial ATPase and cross-resistance to inhibitors of phosphoryl transfer reactions. Molec. Gen. Genet. 178, 351–355 (1980). https://doi.org/10.1007/BF00270483
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DOI: https://doi.org/10.1007/BF00270483