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Isolation and characterization of dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12

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Summary

Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB suppresses the Vrm- phenotype of dam-3 recB21 recC22 strains.

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Authors and Affiliations

  1. Department of Pharmacology, University of Massachusetts Medical School, 01605, Worcester, MA, USA

    Bonnie R. McGraw & M. G. Marinus

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  1. Bonnie R. McGraw
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  2. M. G. Marinus
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Communicated by A.J. Clark

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McGraw, B.R., Marinus, M.G. Isolation and characterization of dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12. Molec. Gen. Genet. 178, 309–315 (1980). https://doi.org/10.1007/BF00270477

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  • DOI: https://doi.org/10.1007/BF00270477

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