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High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12

Construction and characterization of recombinant strains with suppressor-enhancing mutations

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Summary

A set of eight strains combining the supD43,74 ts1 suppressor gene with alleles of three suppressor-enhancing (sue) genes have been constructed and characterized. The sue mutations work cooperatively to raise suppressor activity and together raise the activity of the supD43, 74-encoded suppressor 40-fold. These strains further expand the utility of the ts suppressor system by providing as much as 100% suppressor activity at temperatures at or below 20°C to as little as 0.015% suppressor activity at 43°C.

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Authors and Affiliations

  1. Department of Microbiology, Georgetown University School of Medicine, 20007, Washington, D. C, USA

    Max P. Oeschger & Grant T. Wiprud

  2. Georgetown University School of Dentistry, 20007, Washington, D.C., USA

    Max P. Oeschger & Grant T. Wiprud

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  1. Max P. Oeschger
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  2. Grant T. Wiprud
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Communicated by E. Bautz

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Oeschger, M.P., Wiprud, G.T. High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12. Molec. Gen. Genet. 178, 293–299 (1980). https://doi.org/10.1007/BF00270475

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  • DOI: https://doi.org/10.1007/BF00270475

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