Summary
We have isolated the mutants, T55s-41(a) and T562s-161 (α) which have no sexual agglutinability when cultured at 28°C, but become sexually agglutinable by the action of the sex pheromone produced by respective opposite mating type. The sex-specific glycoproteins responsible for sexual agglutination were detected in the mutants treated with the opposite mating type pheromone, but not in those treated with the same mating type pheromone.
The induction of sexual agglutinability by the pheromone required both nitrogen and carbon sources and was inhibited by cycloheximide. The induction by the pheromone of sexual agglutinability was much more sensitive to osmotic shock and Triton X-100 in T55s-41 than in H1-0, an inducible a strain found in our stock cultures. When cultured at 22°C both T55s-41 and T562s-161 produced respective agglutination substances without the sex pheromones.
H1-0 carried more than one genes responsible for the inducibility (inducible genes). The inducible genes carried by T55s-41 and T562s-161 were recessive, possibly linked to none of the mating type locus, thr4 and his 4, and shown to be identical. The inducible gene in H22, an inducible a strain found in our stock cultures and at least one of the inducible genes in H1-0 were linked to the mating type locus. All the inducible genes observed so far were not specific to the mating type in their action.
Similar content being viewed by others
References
Doi, S., Yoshimura, M.: Temperature-dependent conversion of sexual agglutinability in Saccharomyces cereviseae. Mol. Gen. Genet. 162, 251–257 (1978)
Gunge, N., Tamaru, A.: Genetic analysis of products of protoplast fusion in Saccharomyces cerevisiae. Jap. J. Genet. 53, 41–49 (1978)
Hagiya, M., Yoshida, K., Yanagishima, N.: The release of sexspecific substances responsible for sexual agglutination from haploid cells of Saccharomyces cerevisiae. Exp. Cell Res. 104, 263–272 (1977)
Hereford, L.M., Hartwell, L.H.: Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis. J. Mol. Biol. 84, 445–461 (1974)
Johnston, J.R., Mortimer, R.K.: Use of snail digestive juice in isolation of yeast spore tetrads. J. Bacteriol. 78, 292 (1959)
Mortimer, R.K., Hawthorne, D.C.: Genetic mapping in Saccaromyces. Genetics 53, 165–173 (1966)
Roman, H., Phillips, M.M., Sands, S.M.: Studies of polyploid Saccharomyces. I. Tetraploid segregation. Genetics 40, 546–561 (1955)
Sakai, K., Yanagishima, N.: Mating reaction in Saccharomyces cervisiae. II. Hormonal regulation of agglutinability of a type cells. Arch. Mikriobiol. 84, 191–198 (1972)
Sakurai, A., Tamura, S., Yanagishima, N., Shimoda, C.: Structure of the α peptidyl factor inducing sexual agglutination in Saccaromyces cerevisiae. Agr. Biol. Chem. 40, 1057–1058 (1976)
Sakurai, A., Tamura, S., Yanagishima, N., Shimoda, C.: Isolation of a peptidyl factor, α substance-IA, inducing sexual agglutinability in Saccharomyces cerevisiae. Agr. Biol. Chem. 41, 387–393 (1977a)
Sakurai, A., Tamura, S., Yanagishima, N., Shimoda, C.: Structure of a peptidyl factor, α substance-IA, inducing sexual agglutinability in Saccharomyces cerevisiae. Agr. Biol. Chem. 41, 395–398 (1977b)
Sherman, F.: Use of micromanipulator in yeast studies. In: Methods in cell biology, vol. XI (Prescott, ed.), pp. 189–199. (1975)
Shimoda, C., Osumi, M., Yanagishima, N.: Responsiveness of yeast cells to auxin in relation to cell age. Plant and Cell Physiol. 12, 581–592 (1971)
Shimoda, C., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. IV. Retardation of deoxyribonucleic acid synthesis. Physiol. Plant. 29, 54–59 (1973)
Shimoda, C., Yanagishima, N., Sakurai, A., Tamura, S.: Mating reaction in Saccharomyces cerevisiae. IX. Regulation of sexual cell agglutinability of a type cells by sex factor produced by α type cells. Arch. Microbiol. 108, 27–33 (1976)
Shimoda, C., Yanagishima, N., Sakurai, A., Tamura, S.: Induction of sexual agglutinability of a mating-type cells as the primary action of the peptidyl sex factor from α mating-type cells in Saccharomyces cerevisiae. Plant and Cell Physiol. 19, 513–517 (1978)
Sipiczki, M., Ferenczy, L.: Protoplast fusion of Schizosaccharomyces pombe auxotrophic mutants of identical mating type. Mol. Gen. Genet. 151, 77–81 (1977)
Stötzler, D., Kiltz, H.-H., Duntze, W.: Primary structure of α factor peptides from Saccharomyces cerevisiae. Eur. J. Biochem. 69, 397–400 (1976)
Tohoyama, H., Hagiya, M., Yoshida, K., Yanagishima, N.: Regulation of the production of the agglutination substances responsible for sexual agglutination in Saccharomyces cerevisiae: Changes associated with conjugation and temperature shift. Mol. Gen. Genet. 174, 269–280 (1979)
Tsuboi, M., Yanagishima, N.: Effect of cyclic AMP, theophylline and caffeine on the glucose repression of sporulation in Saccharomyces cerevisiae. Arch. Mikrobiol. 93, 1–12 (1973)
Yanagishima, N.: Sexual cell agglutination in Saccharomyces cerevisiae; Sexual cell recognition and its regulation. Bot. Mag. Tokyo Special Issue 1, 61–81 (1978)
Yanagishima, N., Yoshida, K., Hamada, K., Hagiya, M., Kawanabe, Y., Sakurai, A., Tamura, S.: Regulation of sexual agglutinability in Saccharomyces cerevisiae of a and α types by sex-specific factors produced by their respective opposite mating types. Plant and Cell Physiol. 17, 439–450 (1976)
Yoshida, K., Hagiya, M., Yanagishima, N.: Isolation and purification of the sexual agglutination substance of mating type a cells in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 71, 1085–1094 (1976)
Yoshida, K., Yanangishima, N.: Intra-and intergeneric mating behaviour of ascosporogenous yeasts. I. Quantitative analyis of sexual agglutination. Plant and Cell Physiol. 19, 1519–1533 (1978)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Yanagishima, N., Nakagawa, Y. Mutants inducible for sexual agglutinability in Saccharomyces cerevisiae . Molec. Gen. Genet. 178, 241–251 (1980). https://doi.org/10.1007/BF00270469
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270469