Summary
A model is proposed for the nature of the transfer inhibitor of F-like plasmids, and is shown to be applicable to six of these. In this model, the transfer inhibitor has two components. One, the product of a gene called fin (previously fi or i) is relatively non-specific. The other, called a P product, can be recognised by its slow synthesis or action in a newly-infected cell, and is relatively specific. An approximate location for traP, the cistron coding for the P product of Flac, has been found.
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Achtman, M., Willetts, N. S., Clark, A. J.: Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterisation of transfer deficient mutants. J. Bact. 106, 529–538 (1971).
Achtman, M., Willetts, N. S., Clark, A. J.: A conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. J. Bact. 110, 831–842 (1972).
Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, P. E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966).
Dennison, S.: Naturally occurring R factor, derepressed for pilus synthesis, belonging to the same compatibility group as the sex factor F of Escherichia coli K12. J. Bact. 109, 416–422 (1972).
Egawa, R., Hirota, Y.: Inhibition of fertility by multiple drug-resistance factor in E. coli K12. Jap. J. Genet. 37, 66–69 (1962).
Finnegan, D. J., Willetts, N. S.: Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Molec. gen. Genet. 111, 256–264 (1971).
Fredericq, P.: The recombination of colicinogenic factors with other episomes and plasmids. Ciba Found. Symp. on Bacterial Episomes and Plasmids. pp. 163–174 (1969).
Frydman, A., Meynell, E.: Interaction between de-repressed F-like R factors and wild type colicin B factors: superinfection immunity and repressor susceptibility. Genet. Res. 14, 315–332 (1969).
Grindley, N. D. F., Anderson, E. S., Smith, H. R., Grindley, J. N.: The effects of S. typhimurium on derepressed mutants of F-like factors. Genet. Res. 17, 89–93 (1971).
Ippen, K., Achtman, M., Willetts, N. S.: A deletion map of the E. coli K 12 sex factor F: the order of eleven transfer cistrons. J. Bact. 110, 857–863 (1972).
Kahn, P. L., Helinski, D. R.: Relationship between colicinogenic factors E1 and V and an F factor in E. coli. J. Bact. 88, 1573–1579 (1964).
Lawn, A. M., Meynell, E.: Serotypes of sex pili. J. Hyg. (Caub.) 68, 683–694 (1970).
Lawn, A. M., Meynell, G. G., Meynell, E., Datta, N.: Sex pili and the classification of sex factors in the Enterobacteriaceae. Nature (Lond.) 216, 343–346 (1967).
MacFarren, A. C., Clowes, R. C.: A comparative study of two F-like colicin factors, ColV2 and ColV3, in Escherichia coli K 12. J. Bact. 94, 365–377 (1967).
Meynell, E., Cooke, M.: Repressor-minus and operator constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC. Genet. Res. 14, 309–313 (1969).
Meynell, E., Datta, N.: Functional homology of the sex factor and resistance transfer factors. Nature (Lond.) 207, 884–885 (1965).
Meynell, E., Datta, N.: Mutant drug resistance factors of high transmissibility. Nature (Lond.) 214, 885–887 (1967).
Meynell, E., Meynell, G. G., Datta, N.: Phylogenetic relationships of drug resistance factors and other transmissible bacterial plasmids. Bact. Rev. 32, 55–83 (1968).
Negrotti, T., Nagel de Zwaig, R.: Interactions between colicinogenic factor B and F type factors: isolation of a mutant Flac factor insensitive to the effect of colicinogenic factor B. Genetics 70, 205–213 (1972).
Puig, J., Nagel de Zwaig, R.: Étude génétique d'un facteur colicinogène B et son influence sur la fertilité des croisements chez Escherichia coli K 12. Ann. Inst. Pasteur 107 (No 5 suppl.), 115–131 (1964).
Silver, R. P., Cohen, S. N.: Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterisation of R factor mutants exhibiting temperature-sensitive repression of fertility. J. Bact. 110, 1082–1088 (1972).
Watanabe, T., Fukasawa, T.: Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factor and F factor in Escherichia coli K 12. J. Bact. 83, 727–735 (1962).
Watanabe, T., Nishida, H., Ogata, C., Arai, T., Sato, S.: Episome-mediated transfer of drug resistance in Enterobacteriaceae. VII. Two types of naturally occurring R factors. J. Bact. 88, 716–726 (1964).
Willetts, N. S.: Plasmid-specificity of two proteins required for conjugation in E. coli K 12. Nature (Lond.) New Biol. 230, 183–185 (1971).
Willetts, N. S., Achtman, M.: A genetic analysis of transfer by the E. coli sex factor F, using P1 transductional complementation. J. Bact. 110, 843–851 (1972).
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Communicated by R. Devoret
Supported by a George Murray Fellowship from the University of Adelaide, Australia. Present address: Laboratory of Genetics, Department of Biochemistry, University of Oxford.
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Finnegan, D., Willetts, N. The nature of the transfer inhibitor of several F-like plasmids. Molec. Gen. Genet. 119, 57–66 (1972). https://doi.org/10.1007/BF00270444
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DOI: https://doi.org/10.1007/BF00270444