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Sialoglycoderivatives of bovine submandibular gland identified in situ by histochemical techniques combined with lectins

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Summary

We employed sialidase procedures followed by lectin stainings combined with oxidizing and deacetylating agents to visualize the distribution and sequentiate sialoglycoconjugates in the bovine submandibular gland. In particular we evidenced in acinar and ductal cells the dishomogeneous presence of sialic acids acetylated in the polyhydroxy side chain (C7, C8, C9), whereas O-acetyl substituents at position C1 and/or C4 were not found. Sialoglycoderivatives were also differentiated by the occurrence of penultimate sugars; indeed the dimers sialic acid-(α2→3,6)-β-galactose and sialic acid-(α2→6)-α-N-acetylgalactosamine were identified. Using such technique we supported further the possibility to develop methods for the identification of the positions of Oacetyl groups and the reconstruction of terminal disaccharides within surface and cytoplasm glycoconjugates.

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Authors and Affiliations

  1. Dipartimento di Biologia molecolare, cellulare e animale, Università di Camerino, Via A. Moro 10, I-62032, Camerino (MC), Italy

    G. Menghi, D. Accili, P. Scocco & G. Materazzi

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  1. G. Menghi
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  2. D. Accili
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  3. P. Scocco
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  4. G. Materazzi
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Menghi, G., Accili, D., Scocco, P. et al. Sialoglycoderivatives of bovine submandibular gland identified in situ by histochemical techniques combined with lectins. Histochemistry 97, 397–403 (1992). https://doi.org/10.1007/BF00270386

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  • DOI: https://doi.org/10.1007/BF00270386

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