Summary
A strain of Escherichia coli, VT, which spontaneously gives rise to mutations in many ribosomal proteins, has been used in conjunction with chemical mutagenesis and varying the subsequent incubation temperature to select mutants which have alterations in every ribosomal protein amenable to analysis of 70 S proteins on two-dimensional polyacrylamide gels under standard conditions. Alterations have been detected in 50 ribosomal proteins, namely in 20 from the small and in 30 from the large subunit. This is the most complete set of mutants with altered ribosomal proteins described so far. The difficulty until recently in obtaining mutations in most ribosomal proteins arises not because they are lethal, as has often been supposed, but because of the lack of a suitable selection heretofore.
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Adelberg, E.A., Mandel M., Chen, G.C.C.: Optimal conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem. biophys. Res. Comm. 18, 788–795 (1965)
Dabbs, E.R., Wittmann, H.G.: A strain of Escherichia coli which gives rise to mutations in a large number of ribosomal proteins. Molec. gen. Genet. 149, 303–309 (1976)
Guerola, N., Ingraham, J.L., Cerda-Olmedo, E.: Induction of closely linked multiple mutations by nitrosoguanidine. Nature (Lond.) New Biol. 230, 122–125 (1971)
Hasenbank, R., Guthrie, C., Stöffler, G., Wittmann, H.G., Rosen, L., Apirion, D.: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 127, 1–18 (1973)
Isono, K., Krauss, J., Hirota, Y.: Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Molec. gen. Genet. 149, 297–302 (1976)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins, VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970)
Pettersson, I., Hardy, S.J.S., Liljas, A.: The ribosomal protein L8 is a complex of L7/L12 and L 10. FEBS Lett. 64, 135–138 (1976)
Wittmann, H.G., Stöffler, G., Hindennach, I., Kurland, C.G., Randall-Hazelbauer, L., Birge, E.A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R.R., Bickle, T.A.. Correlation of 30 S ribosomal proteins of Escherichia coli isolated in different laboratories. Molec. gen. Genet. 111, 327–333 (1971)
Wittmann, H.G.: Purification and identification of Escherichia coli ribosomal proteins. In: Ribosomes (Nomura, M., Tissières, A., Lengyel, P., eds.), pp. 93–114: Cold spring Harbour Lab. Monogr. Series, 1974
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Communicated by E. Bautz
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Dabbs, E.R. Mutational alterations in 50 proteins of the Escherichia coli ribosome. Molec. Gen. Genet. 165, 73–78 (1978). https://doi.org/10.1007/BF00270378
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DOI: https://doi.org/10.1007/BF00270378