Rheumatology International

, Volume 7, Issue 2, pp 67–70 | Cite as

T cell subsets in the blast cell population in phytohemagglutinin-stimulated peripheral blood in vitro and in rheumatoid arthritis synovial fluid in vivo

  • D. Nordström
  • Y. T. Konttinen
  • V. Bergroth
  • M. Segerberg-Konttinen
  • S. Santavirta


The immunomodulatory T4/T8 ratio was studied in the total and activated lymphocyte populations by a method combining visualisation of 3H-thymidine incorporating blasts with autoradiography (AR) with simultaneous identification of the respective lymphocyte subsets using monoclonal antibodies in avidin-biotin-peroxidase complex (ABC) staining. In rheumatoid arthritis synovial fluid the activated T4/T8 ratio (calculated from T cells in the S phase of the cell cycle) was significantly different from the total T4/T8 ratio (calculated for all the T cells) (0.45±0.05 versus 0.69±0.05, P<0.01). Similarly, the activated and total T4/T8 ratios were also significantly different in the phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cell cultures at days 3 and 5.

Key words

Rheumatoid arthritis Synovial fluid Lymphocyte activation Immunoregulation 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • D. Nordström
    • 1
  • Y. T. Konttinen
    • 1
  • V. Bergroth
    • 1
  • M. Segerberg-Konttinen
    • 1
  • S. Santavirta
    • 1
  1. 1.Fourth Department of MedicineUniversity Central HospitalHelsinkiFinland

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