Abstract
An improved technique is described for the culture of explants from Helianthus tuberosus L. (Jerusalem artichoke). No xylem is formed when tuber discs are pre-cultured on a medium containing ßNAA, allowing uninfected discs to be selected for investigation of xylogenesis. Subsequent growth on a medium containing 0.45μM 2,4-D and 9.3μM kinetin stimulates a high proportion (up to 36%) of the cells to differentiate into xylem elements within a relatively short time (between 1 and 3 d after transfer).
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References
Fukuda H, Komamine A (1980) Plant Physiol 65: 57–60.
Gamborg O L, Miller R.A, Ojima K (1968) Exp Cell Res 50: 151–158.
Haddon L, Northcote D.H (1976) Planta 128: 255–262.
Minocha S C, Halperin W (1974) Planta 116: 319–331.
Minocha S C, Halperin H (1976) Can J Bot 54: 79–89.
Phillips R (1980) Int Rev Cyt Supplement 11A: 55–70.
Torrey J G, Fosket D E, Hepler P K (1971) Am Sci 59: 338–352.
Watson B, Halperin W (1981) Z Pflanz 101: 145–158.
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Markland, W., Haddon, L. An improved technique for the culture of jerusalem artichoke (Helianthus tuberosus L.) explants for use in the study of xylem differentiation. Plant Cell Reports 1, 229–231 (1982). https://doi.org/10.1007/BF00270243
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DOI: https://doi.org/10.1007/BF00270243