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In vitro synthesis of lectins in cell-free extracts from dry wheat and rye embryos

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Abstract

Cell-free extracts from dry wheat (Triticum vulgare L.) and rye (Secale cereale L.) embryos do not synthesize their corresponding lectins when incubated under conditions optimalized for translation of either exogenous or endogenous mRNA. Only when the extracts are prepared and incubated in the complete absence of sulphydryl containing compounds lectins are synthesized in vitro. Since immunoprecipitation techniques could not demonstrate unequivocally the presence of lectin among the cell-free translation products a specific affinity purification procedure has been developed in order to proof the presence of stored lectin mRNAs in dry wheat and rye embryos.

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Abbreviations

DTT:

dithiothreitol

SDS-PAGE:

sodium dodecyl sulphate polyacrylamide gel electrophoresis

TCA:

trichloracetic acid

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Authors and Affiliations

  1. Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000, Leuven, Belgium

    Willy J. Peumans, Hetty M. Stinissen, Mieke Tierens & Albert R. Carlier

Authors
  1. Willy J. Peumans
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  2. Hetty M. Stinissen
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  3. Mieke Tierens
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  4. Albert R. Carlier
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Peumans, W.J., Stinissen, H.M., Tierens, M. et al. In vitro synthesis of lectins in cell-free extracts from dry wheat and rye embryos. Plant Cell Reports 1, 212–214 (1982). https://doi.org/10.1007/BF00270238

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  • DOI: https://doi.org/10.1007/BF00270238

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