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Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.

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Abstract

Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars (“Loras” and “Tower”) of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. They developed to mature embryos when the auxin was reduced or omitted.

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Abbreviations

ABA:

abscisic acid

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

NAA:

1-naphthaleneacetic acid

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Authors and Affiliations

  1. Botanisches Institut der Johann Wolfgang Goethe-Universität, D-6000, Frankfurt am Main, Germany

    Liang-cai Li & Hans Willy Kohlenbach

Authors
  1. Liang-cai Li
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  2. Hans Willy Kohlenbach
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Additional information

Dedicated to Prof. Dr. Wilhelm Halbsguth on the occasion of his 70th birthday.

On leave from the Institute of Genetic, Academia Sinica, Beijing, China

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Li, Lc., Kohlenbach, H.W. Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.. Plant Cell Reports 1, 209–211 (1982). https://doi.org/10.1007/BF00270237

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  • DOI: https://doi.org/10.1007/BF00270237

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