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Biosynthesis of cyclopentenylglycine from α-ketopimelate in Idesia polycarpa callus cultures

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Abstract

The nonproteinogenic amino acid, cyclopentenylglycine, is found in certain Flacourtiaceae. This compound may be synthesized by two C1-chain elongations of α-ketoglutarate via α-ketopimelate (C5+2C1) or by condensation of C4 and C3 units (C4+C3), a pathway not involving α-ketopimelate. The following experimental design elucidated the biosynthetic pathway: Idesia polycarpa callus cultures were freshly established from leaf petioles; synthetic α-[1,2-14C]ketopimelate was added to the medium and cultures were incubated for 3 weeks. After isolation and separation of free amino acids from the tissues, the radioactivity incorporated into cyclopentenylglycine was determined. The results establish α-ketopimelate as a precursor for cyclopentenylglycine, thus providing evidence for the C5+2C1 biosynthetic path.

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Authors and Affiliations

  1. Institut für Biochemie, Universität Münster, Orléansring 23a, D-4400, Münster, Germany

    Ingrid Tober & Friedrich Spener

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  1. Ingrid Tober
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  2. Friedrich Spener
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Tober, I., Spener, F. Biosynthesis of cyclopentenylglycine from α-ketopimelate in Idesia polycarpa callus cultures. Plant Cell Reports 1, 193–196 (1982). https://doi.org/10.1007/BF00270232

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  • DOI: https://doi.org/10.1007/BF00270232

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