Abstract
DNA synthesis in protoplasts isolated from soybean cell suspension cultures has been investigated by [3H] thymidine uptake and incorporation kinetics. Initial rates of incorporation in exponential and 5-fluorodeoxyuridine synchronized protoplasts are inhibited by increased osmolarities of the medium. The inhibition was not readily reversible during 3 h culture in low osmotic medium. Velocity sedimentation analyses of replicating DNA from such protoplasts shows a complex pattern of inhibition. The inhibition probably effects replicon initiation as well as strand elongation and ligation of replication intermediates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chu YE, Lark KG (1976) Planta 132:259–268
Cress DE, Jackson PJ, Kadouri A, Chu YE, Lark KG (1978) Planta 143:241–253
Galun E (1981) Ann. Rev. Plant Physiol. 32:237–266
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell. Res. 50:151–155
Lark KG, Cress DE (1978) In: Thorpe TA (ed) Frontiers of Plant Tissue Culture 1978, Proceedings of the 4th International Congress of Plant Tissue and Cell Culture, Calgary, Canada, pp, 179–189
Lurquin PF (1979) Nucleic Acids Res. 6:3773–3784
Pardoll DM, Vogelstein B, Caffey DS (1980) Cell 19:527–536
Scudiero D, Henderson E, Norin A, Strauss B (1975) Mutat. Res. 29:473–488
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cress, D.E. Osmotic stress inhibits thymidine incorporation into soybean protoplast DNA. Plant Cell Reports 1, 186–188 (1982). https://doi.org/10.1007/BF00270230
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270230